IMOs are mixtures of oligosaccharides in which the Glucose moieties are predominantly linked by α-1,6 glycosidic bonds. As a kind of non-digestible oligosaccharide, IMOs have low glycemic index characteristics, which are conducive to the maintenance of blood glucose homeostasis. IMOs also have physiological functions such as lowering cholesterol levels and alleviating intestinal inflammation, which is widely used in the field of food and health care. A large number of studies and scientific evidence have proved that IMOs have a positive impact on physiological health. IMOs can promote the proliferation of beneficial bacteria such as Bifidobacterium and Lactobacillus after entering the colon. At the same time, IMOs inhibit the growth of harmful intestinal bacteria, as well as the formation of spoilage and harmful substances. IMOs are found to play a therapeutic role in promoting the repair of colonic mucosa and protecting the barrier function of colonic mucosa in UC rats.
CD BioGlyco develops a one-stop analytical service for structural and bioactivity analysis. We provide multi-directional IMO analytical services.
To further understand the properties of IMO, structural characterization of IMO is required. We examine the average molecular weight and degree of polymerization of IMO by high-performance gel filtration chromatography (HPGFC). A simultaneous thermal analyzer is used to determine the thermal stability of IMO. Scanning electron microscopy is employed to observe the surface morphology of IMO. Functional groups are detected by nuclear magnetic resonance technique (1H NMR). We will also test the physicochemical properties of IMO, including water content, pH, solubility, stability, etc.
Fig.1 Route to IMO structural analysis. (CD BioGlyco)
We have extensive experience in testing the effect of Oligosaccharides on the intestinal flora. We will test the effect of IMOs on the growth status of beneficial bacteria, intestinal commensals, etc., and the in vitro fermentation performance of beneficial bacteria. We also conduct in vivo experiments. We will feed mice with different doses of IMOs by tube feeding and analyze the colonies in their feces after a certain number of days. For the therapeutic effect of intestinal inflammation, we will construct mice models of diseased. We will analyze the pharmacological activity of IMOs by comparing the physiological characteristics of the experimental group and the control group after IMOs administration. IMOs are also capable of slowing down the progression of colon cancer precursor lesions. We will construct mouse models of cancer and test the potential use of IMOs in cancer therapy.
Fig.2 IMOs bioactivity assay. (CD BioGlyco)
Paper Title: Immobilization of α-transglucosidase on silica-coated magnetic nanoparticles and its application for the production of isomaltooligosaccharide from the potato peel
Technology: Gas chromatography-mass spectrometry (GC-MS), NMR, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS)
Journal: Scientific Reports
Results: In this study, IMOs were produced from potato peel starch. The IMOs generated were analyzed using MALDI-TOF-MS. The results showed that the polymerization (DP) of the IMOs was in the range of 2-10. The structure of IMO was analyzed by GC-MS and NMR. The results showed that the α-(1→4) and α-(1→6)-D-Glcp residues are the major constituents along with minor α-(1→2) and α-(1→3)-D-Glcp residues.
Fig.3 MALDI-TOF-MS spectrum of IMOs. (Maurya, et al., 2023)
CD BioGlyco is experienced in the analysis of Carbohydrate Metabolism with a dedicated research team. With experience in isolation, characterization, and detection, we offer a wide range of services for metabolic studies, drug discovery, and more. If you are interested in learning more about our testing programs, please feel free to contact us.