Due to the low abundance and ionization efficiency of glycopeptides, the identification strategies of glycopeptides based on mass spectrometry (MS) are facing great challenges. CD BioGlyco focuses on the enrichment process before MS analysis and develops a variety of new materials, including graphene, to provide clients with efficient glycopeptide enrichment and separation services.

Graphene

Efficiently capturing glycopeptides from complex biological samples is essential for glycoproteomic analysis. A variety of novel nanomaterials have been developed for glycopeptide enrichment. Since the Nobel Prize in physics in 2010, graphene has become an increasingly attractive material consisting of single atomic layers of carbon, which has many unique properties that make it interesting and suitable for basic research and future applications. Surface functionalized graphene or graphene oxide is now being used for glycan enrichment because of its large specific surface area and superb absorption capacity.

Researchers have designed and synthesized hydrophilic chitosan-functionalized magnetic graphene nanocomposites (denoted as Fe₃O₄-GO@PDA-chitosan) by a simple two-step modification including dopamine self-polymerization and Michael addition. The resulting nanocomposites showed high selectivity and sensitivity for the enrichment of N-glycopeptides from complex biological samples due to the enhanced hydrophilicity by the PDA layer and chitosan. This report provides a feasible strategy for the separation and enrichment of N-glycopeptides by surface-functionalized novel materials.

Fig.1 Schematic diagram of the procedure for preparation of Fe₃O₄-GO@PDA and Fe₃O₄-GO@PDA-Chitosan and N-glycopeptides enrichment. (Bi, 2020)

Our Strategies

CD BioGlyco has established several strategies for rapid and efficient enrichment of glycans released from glycoprotein, even in the presence of multiple interfering non-glycoproteins, to provide clients with glycomics analysis services. The strategies we offer include but are not limited to:

• We employ a novel hydrophilic composite based on electrostatic interactions that can effectively immobilize cationic cellulose on a graphene oxide-dopamine carrier (denoted as GO-DA-JR) for highly specific enrichment of N-glycopeptides.
• We have developed a technique for rapid, efficient and visualized glycans enrichment using 1-pyrenebutyryl chloride functionalized graphene oxide. The large specific surface area and heavy functionalization of activated 1-pyrenebutyryl chloride greatly improve the enrichment efficiency.
• We have explored a novel polymer hybridized graphene oxide (GO) by in situ growth of hydrazide functionalized hydrophilic polymer chains on the GO surface for N-glycopeptide enrichment and MS identification.

Workflow

Applications

• Our services can be used to elucidate the role of glycosylation in disease pathogenesis, identify and validate new drug targets, and evaluate efficacy.
• Our services enable basic research into the functions of sugars and glycopeptides in cell-cell communication, immune responses, protein folding, and cell signaling pathways.
• Our services can be used to characterize the glycosylation patterns of therapeutic proteins (e.g., monoclonal antibodies, recombinant proteins).

Advantages of Us

• The unique surface chemistry of our graphene materials also contributes to higher recovery rates, minimizing sample loss during the enrichment process.
• Our graphene functionalization ensures superior selectivity and sensitivity for glycopeptide capture, significantly reducing background noise and enhancing the detection of low-abundance glycopeptides.
• We accommodate various sample throughput requirements, from individual samples to large-scale cohort studies, providing flexibility and scalability for your research projects.

Publication Data

Frequently Asked Questions

Researchers of CD BioGlyco have established a variety of glycopeptide enrichment methods with high selectivity, high sensitivity, good stability and operability. We offer high-quality services in the field of glycoproteomics for our clients. If you are interested in our services, please contact us for further consultation.

Associated Services

• Integrated Glycopeptide Identification and Quantification: Beyond enrichment, we offer advanced MS services for the in-depth identification and accurate quantification of enriched glycopeptides. This includes sophisticated data analysis to provide detailed insights into glycan structures and protein glycosylation sites.
• Custom Graphene Functionalization: If your research requires highly specialized enrichment capabilities, our team of experts develops custom-functionalized graphene materials tailored to your specific targets or experimental needs.
• Glycopeptide Microarray: We provide high-quality glycopeptide microarray development services to help clients explore molecular mechanisms in depth.