With years of research and experience in glycoengineering, multiple adverse factors have been tackled and optimized to improve the productivity of glycoprotein. CD BioGlyco is proficient at employing the chemoenzymatic method for sialic acid glycoengineering of glycoprotein. We offer high-quality customized services by adjusting strategies to meet even the most specific requirements in the production and analysis of glycoprotein.

ManNAc Analogs

Sialic acid glycoengineering is increasingly applied in the field of glycoscience and beyond. Chemically modified analogs of N- N-acetylmannosamine (ManNAc), the biological precursor of sialic acid, or sialic acid analogs are introduced into the metabolic sialic acid biosynthesis pathway resulting in their incorporation into sialoglycans.

Over the last two decades, more than 20 unnatural mannosamines have been introduced as suitable substances for metabolic oligosaccharide engineering. These ManNAc analogs can be divided into two groups: aliphatic and bioorthogonal analogs. In aliphatic analogs, the N-acyl function is elongated by one or more methylene groups. Metabolic oligosaccharide engineering with aliphatic ManNAc analogs introduces novel biological characteristics to cells and consequently influences their behavior in multiple ways. Bioorthogonal analogs carry modifications of the N-acyl side chain that are both absent in living systems and utilizable for further chemical reactions. These reactive groups are applied, for example, to label sialylated glycans with a fluorescent dye.

Fig.1 Metabolic glycoengineering using ManNAc analogs carrying biorthogonal modifications. (Wratil, et al., 2016)

ManNAc and Its Analogs-based Sialic Acid Glycoengineering Services

CD BioGlyco is proficient at multi-step chemical or enzymatic synthesis and quantitative analysis techniques for glycoproteins with different sialic acids. Services we provide include but are not limited to:

• Production of glycoproteins with different sialic acids according to customer requirements.
• Chemical modification of cell surface glycans at the N-acyl position.
• Pre-treatment of samples, such as Intracellular esterase deacetylation, biorthogonal reaction to fluorophores, and so on.
• Determination of incorporation and metabolic efficiency of samples using flow cytometry or other techniques.
• Using radioactivity, mass spectrometry, or HPLC for quantitative analysis of the sialic acids on the modified cell surface, such as ManNProp, ManNBut, ManNPent, ManNHex, ManNHept, ManNLev, ManNAcPh, Ac₄ManNAz, Ac₄ManNHomoLev, Ac₄ManNOxoHex, Ac₅ManNGc, Ac₅ManNTGc, Ac₄ManNDAz, and so on.

Advantages of Us

• Outstanding scientists specialize in glycoengineering
• Production of glycoproteins with different sialic acid according to customer requirements
• Ensuring the amount and purity of glycoprotein delivered
• High stability and consistency of experimental results
• Fast, reliable, customized, and meet customer expectations

CD BioGlyco has the world's leading sialic acid glycoengineering technologies. Scientists at CD BioGlyco are fully competent and dedicated to serving as your one-stop service for custom production of glycoprotein with different sialic acids. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Wratil, P.R.; et al. Metabolic glycoengineering with N-Acyl side chain modified mannosamines. Angewandte Chemie International Edition. 2016, 55(33): 9482-9512.