Cell-based Glycosaminoglycan (GAG) Array

Cell-based Glycosaminoglycan (GAG) Array

High-level Cell-based GAG Array That Exceeding Your Expectations

GAGs are highly negatively charged linear polysaccharides. It consists mainly of disaccharide repeats composed of glucose or galactose and hexane-dioic acid. CD BioGlyco has developed different structures of GAG array on cells such as chondroitin sulfate (CS), dermatan sulfate (DS), heparin sulfate (HS), heparin (HP), and keratan sulfate (KS). More than 40 glycosyltransferases, epimerases, sulfotransferases, and other enzymes are involved in the biosynthesis of GAGs. We provide modification services such as sulfation, N-acetylation, and differential isomerization. Meanwhile, utilizing the ability of cells to carry out GAG biosynthesis, our researchers produce and display GAG on a cell-based array and interrogate the detailed structural features required for specific biological activities.

  • Design and construction service
  • Our researchers use precise gene editing services to design Chinese hamster ovary (CHO) cells and human embryonic kidney 293 (HEK293) cells. We have obtained allelic cell libraries showing different GAG characteristics by knocking out or knocking in the GAG biosynthetic enzyme genes. In addition, we provide binding specificity analysis and biosynthesis regulation evaluation of cell libraries.
  • We assemble cell-based GAG arrays through cell libraries. Our researchers have years of experience in Cell-based Glycan Display Array construction to accurately help clients use engineered cells to produce different GAG structure arrays.
  • High-throughput screening service
  • We investigate the role of GAG features on cell binding by flow cytometry and dissect the aberrant expression of GAG epitopes on cells. The analytical service provides both genetic and biosynthetic information. In addition, we provide services on the interaction relationship between cell-based GAG array and pathogens, growth factors, and antibody proteins.

Fig.1 Schematic diagram of cell-based GAG array development service. (CD BioGlyco) Fig.1 Schematic diagram of cell-based GAG array service. (CD BioGlyco)


Paper Title: The GAGOme: a cell-based library of displayed glycosaminoglycans

Technology: RNA sequencing, High-performance liquid chromatography (HPLC), Gene editing, Flow cytometry

Journal: Nature Methods

Published: 2018

IF: 47.99

Results: In this paper, a gene-targeted gRNA library was developed to extend the GAGOme library. A more comprehensive GAG library was constructed from cell lysates or by recombinantly expressed proteoglycans, which was further used to generate cell-based arrays to display and study the biological functions of GAGs. Further refinement of epitope recognition and identification of biosynthetic and genetic bases was achieved by displaying different distinct protein-bound GAGs through the GAGOme library.

Fig.2 Predicted GAG biosynthetic capabilities of WT CHO and HEK293 cells based on RNAseq analysis. (Chen, et al., 2018)Fig.2 Predicted GAG biosynthetic capabilities of WT CHO and HEK293 cells based on RNAseq analysis. (Chen, et al., 2018)

Applications of Cell-based Glycosaminoglycan (GAG) Array

  • Cell-based GAG array dissects the fine-binding specificity of pathogen adhesion, growth factors, and antibodies.
  • Cell-based GAG library can be used for the production of recombinant proteoglycans of GAG chains with different GAG structures and GAG chains.
  • Cell-based GAG array is a potential screening tool for comprehensively exploring the biological function of glycans.

CD BioGlyco has professional operators and sophisticated high-throughput analytical instruments to provide satisfactory service. Our Glycan Display Platform provides a comprehensive service for clients. If you want to know more information, please feel free to contact us.


  1. Chen, Y.H.; et al. The GAGOme: a cell-based library of displayed glycosaminoglycans. Nature methods. 2018, 15(11): 881-888.
This service is for Research Use Only, not intended for any clinical use.

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