CD BioGlyco has rich experience in peptidoglycan structural analysis. Our multiple technology platforms and well-trained researchers can help customers get results quickly and accurately in the case of low-abundance samples. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Peptidoglycan (PG, also known as murein) exists in the cell walls of gram-positive bacteria and gram-negative bacteria in eubacteria. The backbone of peptidoglycan is a polysaccharide chain formed by two sugar derivatives: N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) that alternately connected by β-1,4-glycosidic bonds. Two parallel sugar chains are connected laterally to form a network of peptidoglycan. PG is essential for maintaining cell integrity because of its ability to withstand turgor pressure. Any external force that affects its biosynthesis or degradation will interfere with cell viability. Therefore, PG is one of the main targets of antibacterial drugs. The study of peptidoglycan structure also plays a vital role in understanding the immune response, phage susceptibility and serological behavior, as well as the classification and identification of bacteria. Although the structure and architecture of PG have been studied for a long time, its detailed 3D structure is still an open question.

Fig 1. Cell wall structure of Gram-positive and Gram-negative bacteria (Porfírio, S.; et al. 2019)

Services

CD BioGlyco provides a one-stop service to assist in studies from PG separation to structural analysis, and each process is strictly controlled by experienced researchers. The experimental operations are built on our multiple technology platforms, including but not limited to:

• Amino acid analyzers
• Thin layer chromatography (TLC)
• High-pH anion-exchange chromatography (HPAEC)
• High-performance liquid chromatography/ultra-performance liquid chromatography HPLC/UPLC-MS
• High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)
• Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)
• Ion trap mass spectrometry (Q-Trap MS)
• Electrospray ionization-mass spectrometry (ESI-MS)
• Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
• Nuclear magnetic resonance (NMR)
• Cryotransmission electron microscopy/Atomic force microscopy (cryo-TEM/AFM)

Fig 2. Flow chart of peptidoglycan structure analysis - CD BioGlyco

Applications

• Cell structure and integrity research
• Development of antimicrobial drugs
• Microbiology and agriculture research
• Mechanism research

Advantages of Us

• Advanced and diverse technology platforms
• Stability and consistency
• One-stop service
• Cost-effective

CD BioGlyco provides systematic and one-stop services for peptidoglycan structure research and ensure the continuity and transparency of the experimental process. We will continue to improve our standards to meet customers' glycobiology research needs with high quality.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Porfírio, S.; et al. Elucidating Peptidoglycan Structure: An Analytical Toolset. Trends in Microbiology. 2019, 277(7): 607-622.