The aerobic glycolysis capacity of cancer cells is 20-30 times that of normal cells, and the enhancement of glycolysis promotes the malignant progression of cancer. The main way to enhance glycolysis in cancer cells is to adjust glycolytic enzymes to enhance their activity and overexpress them. Thus, proteomic analysis of cancer cell glycolysis mainly involves the expression, activity, and interactions of enzymes at various steps in the glycolytic pathway. Enzymes abnormally expressed in cancer cells have become targets for antitumor therapy. CD BioGlyco provides quantitative analysis of enzymes in each step of the glycolytic pathway based on proteomics technology, analysis of post-translational modifications, and analysis of interactions.
Fig. 1 The flowchart of glycolytic (Keighren MA, et al., 2016)
The glycolytic pathway contains numerous reversible enzymatic reactions and three irreversible enzymatic reactions. Changes in the enzymatic response of the glycolytic pathway in cancer cells are involved in the metabolic reprogramming of cancer. We can provide quantification, modification analysis, and related protein interaction analysis services for the following enzymes.
Glyceraldehyde 3-phosphate dehydrogenase
The level of glycolysis in cancer cells is regulated by a network of multiple factors, and analyses targeting single proteins did not capture the complexities of metabolic reprogramming in cancer. We provide comprehensive proteomic analysis of cancer glycolytic metabolic pathways with the following advantages:
Our services can support the following applications, including but not limited to:
CD BioGlyco can help customers analyze the quantitative expression of all enzymatic reaction enzymes in the glycolytic pathway, post-translational modifications, and interactions with other proteins. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.