Application of Glycan Microarrays in Glycan Analysis

Microarray technology is a high-throughput (HT) experimental technique for the simultaneous detection and analysis of multiple biomolecules in a large number of samples. It works by immobilizing thousands of different probe molecules on a solid surface, to create a small array. These probes can be DNA, RNA, proteins, antibodies, glycan, and more. Each probe location has a unique identifier, enabling accurate identification and analysis of which molecules bind specifically to the corresponding probe. Glycan microarrays involve immobilizing numerous different sugar molecules onto a microarray which are bound to specific probes to detect reactions. This technique is capable of simultaneously detecting the conjugation of multiple sugars to the probe, thus providing the ability to HT screen and characterize specific sugar-binding partners. A wide range of natural glycan structures can be covered in Glycan Microarrays, allowing a comprehensive understanding of the interactions between glycans and other biomolecules.

Fig.1 Microarray-based technologies for studies of biological processes. (Shin, et al., 2005)

Glycan Microarrays-based HT Glycan Screening Service at CD BioGlyco

CD BioGlyco has established an advanced Glycobiology Microarray Platform, which provides specialized HT Glycan Screening Services based on glycan microarray assay. We simultaneously test the interaction of a large number of glycans of different structures with target molecules, opening up entirely new possibilities for your glycobiology research. Our HT glycan screening services are listed below:

• Analysis of glycan-protein binding
  We provide our clients with rapid, HT analysis of the conjugation relationship among sugars as well as proteins. Different glycan molecules are immobilized on the microarray and then protein samples are added to react with the glycan molecules. Then, parameters such as binding affinity and specificity between sugars and proteins are assessed by relevant assays.
• Analysis of specific recognition of glycans
  A diverse range of sugar molecules is displayed on the microarray and interacts with biomolecules of interest. These biomolecules can be proteins, receptors, or other binding chaperones. We perform HT detection of biomolecule interactions with various sugar molecules on the microarray to evaluate their specific recognition.

Fig.2 Process of glycan-protein binding analysis based on glycan microarrays. (CD BioGlyco)

Applications

• Studying interactions among glycans as well as proteins
• Vaccine research and development
• Glycan modification

Advantages of Us

• Real-time monitoring of interactions
• Strict quality control and compliance standards
• Quick response and delivery

CD BioGlyco provides glycan microarrays-based HT glycan screening service to help clients accurately assess the binding affinity and specificity of candidate glycans to target proteins. Please feel free to contact us if you are interested in our service, and we will be delighted to provide you with support and assistance.