Abrus precatorius lectin (APA) is a natural lectin derived from jequirity beans. The seeds of jequirity beans contain nontoxic lectins and several toxic components that allow isolation using a variety of conventional techniques. APA has a variety of biological functions, including recognizing and binding specific glycosyl sequences, promoting cell aggregation, immunogenicity, and anti-tumor activity. CD BioGlyco provides professional Lectin Production Services, including Natural Lectin Production Service, Recombinant Lectin Production Service, and Lectin Purification Service.

APA Extraction and Isolation Service at CD BioGlyco

The general extraction and isolation processes of APA are as follows. Firstly, soak the seeds of the jequirity bean in the water and stir it with a mixer. Then filter the suspension solution and collect it. Next, evaporate the filter fluid to remove most of the moisture. Finally, add an appropriate amount of solvents to the concentrated solution, so that the APA is precipitated in the solution.

APA Purification Service at CD BioGlyco

Gel-filtration Chromatography-based Lectin Purification and Ion Exchange Chromatography-based Lectin Purification technologies are mainly used in the purification process. Before chromatographic purification, the precipitate is dissolved in an amount of water to form a crude extract solution of APA.

• Ion-exchange chromatography: Use ion exchanges to exchange the APA crude extract solution to remove impurities. According to the types and concentrations of ion exchanges, the APA is further purified through multiple exchanges.
• Gel-filtration chromatography: Using column layer analysis technology, the APA crude extract solution passes through the gel column. Separate the APA and other impurities according to the difference between the molecular size.

APA Identification Service at CD BioGlyco

• Biological test: Use biochemistry methods (such as ELISA) to detect the concentration and activity of APA. The interaction between APA and specific antibodies are detected to evaluate the quality and purity of APA.
• Molecular measuring detection: Use molecular sieve or gel electrophoresis technology to determine the molecular weight of the APA. The purified APA showes a single, clear band, and its molecular weight is consistent with the expected molecular weight.
• Glycosyl group analysis: The type and proportion of the glycosyl group in APA are measured by gas chromatography or high-performance liquid chromatography.

Fig.1 Steps of APA extraction and isolation. (CD BioGlyco)

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Applications of APA Lectin

• APA is used in western blot analysis to detect and identify specific sequences in proteins.
• APA is used to study the processes of cell differentiation and apoptosis. It identifies and marks glycosyl expression on the cell surface through specific binding to glycosyl groups, thereby helping researchers understand the growth and death mechanisms of cells.
• APA is used for virus detection and identification. It identifies and labels viral particles by binding to sugar groups on the virus surface, thereby helping researchers determine the type and characteristics of the virus.

Advantages of Us

• We have a professional R&D team that ensures product quality and provides customized services.
• We focus on innovative research and continuously invest in exploring new technologies to meet client's needs and maintain market competitiveness.
• We focus on product quality and ensure product reliability.

CD BioGlyco provides a high-quality APA production service that offers a comprehensive solution for the extraction and purification of this valuable lectin. The service incorporates a range of advanced Plant Lectin Production techniques to ensure the quality of the final product. Please do not hesitate to contact us for more details if you are interested in our APA production services.