SLNnH II can be made in a variety of ways, including chemical synthesis, biosynthesis, enzymatic synthesis, extraction from natural sources, and chemical synthesis.

Introduction of SLNnH II Production Methods

Two recent studies found that the location and strength of fucosylation and sialylation play a crucial role in the antimicrobial activity of human milk oligosaccharides (HMOs) and that more complex fucosylated and sialylated HMOs had a greater antimicrobial effect on specific group B streptococcus strains than fucosylated or sialylated lactose. For the synthesis of sialylated HMOs such as 3'-sialyllactose (3'-SL), 6'-sialyllactose (6'-SL), and disialyllacto-N-tetraose (DSLNT), sialyltransferases (SiaTs) of variable specificity have been used.

The number of protective group manipulations, which rises with chain length and branching, is the fundamental obstacle in the chemical synthesis of HMOs. Chemical synthesis as a result frequently takes a long time and produces little. Furthermore, hazardous reagents and often transition metal catalysts are used in chemical synthesis. Hence, for the large-scale synthesis of HMOs, the chemical synthesis of complex carbohydrates is frequently not a manufacturing technique that is cost-effective. Human Leloir glycosyl transferases (GTs), which are active on sugar-nucleotide donor substrates, produce HMOs in vivo. As GTs are often very regio- and stereospecific, they can synthesize glycosides precisely and effectively in a single process. Although there aren't many HMOs now available through cell manufacturing, this is a promising path.

Fig.1 The structure of SLNnH II, also called N-Acetylneuraminyllacto-N-neohexaose. (CD BioGlyco)

SLNnH II Production Services at CD BioGlyco

Normally, SLNnH II can be produced by four methods: extraction from natural sources, Chemical Synthesis, Biosynthesis, and Enzymatic Synthesis. Extraction is the most used method to produce SLNnH II, modifying from other subunits is also a possible way to synthesize it. CD BioGlyco has a powerful synthesis platform, which can provide the production service of SLNnH II with different methods. In addition, CD BioGlyco provides HMO Analysis Services for clients to help them research the field of HMOs, it will help our clients to study the potential application of SLNnH II.

Fig.2 Different methods for SLNnH II production. (CD BioGlyco)

Advantages of Us

• Based on clients' needs, we will produce different amounts of SLNnH II with different production methods.
• We establish an outstanding synthetic platform to produce high-quality SLNnH II.
• According to the client's needs, we can provide different grades of SLNnH II.
• Our professional scientists and service team will provide suitable solutions for our clients' inquiries.

CD BioGlyco has a professional production lab and much production experience. We have the ability to produce different scales and grades of SLNnH II. If you are interested in our SLNnH II production service, please feel free to contact us for more detailed information.

Reference

1. Hussain, M.I.; et al. Enzymatic production of N-acetylneuraminic acid: Advances and perspectives. Systems Microbiology and Biomanufacturing. 2021, 2(1): 130–146.