Amylopectin Chain Length Distribution Profiling
The chain length distribution of amylopectin is directly related to its structure and function. CD BioGlyco provides advanced analysis techniques to assist with amylopectin chain length distribution profiling. We have confidence to be your essential research assistant in the field of glycobiology.
Starch is a polymer containing two types of molecules: amylopectin (AP) and amylose (AM). In ordinary starch granules, AP accounts for about 80%, and AM accounts for about 20%. AP is a highly branched polymer, which is very complex and has a relatively large molecular weight of ~107-8. In AP, the D-glucopyranose unit is linked by α-1,4-glycosidic bonds to form a straight chain. This straight chain can form a side chain through α-1,6-glycosidic bonds, and another side chain will appear on this side chain. Chain length distribution (CLD) is the first structural level among the six identifiable levels of starch, involving individual chains at branch points.
The molecular structure of starch is an important structural feature of the structure-characteristic relationship in starch foods. The starch gelatinization properties are mainly controlled by AP CLD. It has been confirmed that there is a negative correlation between the ratio of short AP branches and the gelatinization temperature, and CLD can also provide information about starch synthase activity. Measuring the CLD of starch molecules can better explore the relationship between its molecular structure and function.
Fig. 1 A schematic representation of amylose and amylopectin, and the structures adopted by the constituent chains (Zeeman, S.C.; et al. 2010.)
Our methodology employs an integrated array of sophisticated technological processes to generate precise chain length distribution profiles with high resolution. Central to our technique is the mild yet exhaustive enzymatic debranching of amylopulin, achieved through the application of enzymes exhibiting stringent specificity—such as isoamylase or pullulanase. This treatment produces a homogeneous ensemble of linear glucans, which are subsequently resolved and quantitatively analyzed using our advanced separation methodologies.
We employ HPAEC with pulsed amperometric detection (PAD) for superior resolution. This method provides a highly detailed chromatogram of the debranched amylopectin chains, allowing for the precise quantification of individual chains up to a high degree of polymerization (DP). This technique is a gold standard for its high sensitivity and ability to resolve subtle variations in chain length distribution.
For samples requiring exceptional sensitivity or for applications with limited material, we utilize FACE. This method involves labeling the reducing ends of the debranched glucan chains with a fluorescent tag, followed by separation using capillary electrophoresis. The high sensitivity of FACE, which has been shown to be compatible with analysis down to single starch granules, makes it ideal for precious or minute samples.
For a broader perspective on the molecular weight of starch fractions, we use SEC. This technique separates molecules based on their hydrodynamic volume, providing a general overview of the size distribution of both amylose and amylopectin, which complements the detailed insights from HPAEC and FACE.
Our amylopectin chain length distribution profiling service provides a comprehensive, high-resolution analysis of this critical molecular feature. By combining advanced enzymatic debranching with state-of-the-art analytical techniques, we offer unparalleled insights into the fine structure of starch, empowering researchers and product developers to optimize their materials for improved performance and quality.
Upon receipt, your starch sample is meticulously prepared to ensure the complete dissolution of starch granules. This is a critical step to make the amylopectin accessible for subsequent enzymatic action.
The gelatinized starch is treated with a specific debranching enzyme to hydrolyze the α-1,6 glycosidic linkages, converting the branched amylopectin molecule into its constituent linear chains.
The resulting linear glucans are then subjected to our high-resolution analytical platforms, such as HPAEC or FACE. This step precisely separates the glucan chains based on their degree of polymerization.
The separated chains are detected and quantified, producing a comprehensive chromatogram or electropherogram that reveals the percentage distribution of each chain length.
The raw data is meticulously analyzed to provide a clear, actionable report detailing the full amylopectin chain length distribution. We correlate these findings with the known physical properties of starch to provide context and guidance for your project.
DOI.: 10.1016/j.carbpol.2024.122264
Journal: Carbohydrate Polymers
IF: 12.5
Published: 2024
Results: The authors investigated how amylopectin chain-length distributions in seven waxy rice varieties influence starch digestibility and pasting properties. Using SEC, they characterized amylopectin chain-length distributions and fitted them to a biosynthesis-based model to derive parameters (β and h) reflecting enzymatic activities. In vitro digestibility assays revealed that shorter chains in amylopectin's long-chain region (βAp,5) correlated with slower digestion rates and higher slowly digestible starch content. Rapid Visco-Analyzer (RVA) profiling showed amylopectin short chains (hAp,1) increased breakdown viscosity (correlation: 0.801), while long chains (hAp,5) reduced peak viscosity. These findings provide a mechanistic basis for breeding waxy rice with optimized digestibility (e.g., lower glycemic response) and desirable cooking textures, linking molecular structure to functional traits in amylose-free starches.
Optimize the texture, mouthfeel, and shelf life of food products such as bakery goods, noodles, and confectionery. Control starch gelatinization and retrogradation to achieve desired product characteristics.
Identify and select new plant cultivars with superior starch properties. Understand the genetic basis of starch biosynthesis by correlating gene expression with amylopectin structure.
Characterize starch substrates for biofuel production, fermentation processes, and the development of biodegradable materials.
Analyze starches used as excipients or functional ingredients to ensure consistent performance and digestibility in drug delivery systems and health supplements.
Our amylopectin chain length distribution profiling service provides critical insights into starch fine structure, enabling precise functional characterization for various applications. Building on this analytical foundation, we extend our expertise to Pharmaceutical Excipient Analysis Service, delivering a comprehensive evaluation of key formulation components to ensure their compliance and performance in drug products.
CD BioGlyco designs customized solutions according to the different characteristics of samples, our advanced equipment and well-trained staff can provide guarantee for customers' starch profiling. We will continue to improve our service quality to better meet customer needs.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.
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