Arum Maculatum Lectin Production Service

Arum maculatum Lectin (AMA) Production Services at CD BioGlyco

CD BioGlyco has long been engaged in research on the various types of Lectins Production, including but not limited to Plant Lectins and Animal Lectins. Among plant lectins, AMA has complex specificity and also interacts with complex N-glycans. At CD BioGlyco, we offer comprehensive AMA production services.

Extraction and purification services

AMA is a monocotyledonous plant lectin, which is extracted and isolated from the tuber of calla lanceolate. We crush the taro tuber, dissolve it in saline, and obtain its crude product through stirring, centrifugation, filtration, and dialysis.

For the purification of AMA, affinity purification of desialic acid fetal globulin by fixed column chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) are excellent ways.

Property testing services
- Structural composition
  The protein composition of the lectin is tested by SDS-PAGE and gel filtration, and the subunit composition of the lectin is tested by sequencing the N-terminus of the protein in combination with the molecular cloning of the lectin.
- Molecular weight
  The total molecular weight of the lectin is identified using polyacrylamide gel electrophoresis, and the molecular weight of its constituent subunits is identified using SDS-PAGE.
- Cross-reactivity
  The cross-reactivity of purified lectin is verified by protein blotting using an AMA antibody.
- Target receptor proteins
  The primary receptor protein for this lectin is detected from brush border membrane vesicle proteins of the intestine using ligand binding. Upon testing, α-d-mannose is found to have a blocking effect on this binding of AMA to the BBMV receptor.
- Coagulation activity assay
  A microplate is taken, and the same concentration of AMA protein is added to each of the four wells, after which different concentrations of rabbit erythrocyte suspensions are injected respectively, and the plate is placed on an oscillator at a medium speed so that the coagulation effect is observed by the naked eye or under a microscope.

Fig.1 AMA production services. (CD BioGlyco)

Currently, we offer the related products as shown in the table below.

Table 1 AMA product list.

Product Name	Product Name
AMA, Lords and ladies, Biotin conjugate	AMA, Lords and Ladies, Colloidal gold conjugate, 10 nm
AMA, Lords and Ladies, Colloidal gold conjugate, 15 nm	AMA, Lords and Ladies, Colloidal gold conjugate, 20 nm
AMA, Lords and Ladies, Colloidal gold conjugate, 30 nm	AMA, Lords and Ladies, Colloidal gold conjugate, 40 nm
AMA, Lords and Ladies, Colloidal gold conjugate, 5 nm	AMA, Lords and ladies, FITC conjugate
AMA, Lords and ladies, Peroxidase conjugate	AMA, Lords and Ladies, Unconjugate
AMA, Lords and ladies, Agarose conjugate	AMA, Lords and ladies, Rhodamine conjugate

Publication

Technology: Models of neutrophil migration in vivo, neutrophil chemotaxis in vitro, and macrophage cultures were used to study the lectin inflammatory activity.

Journal: The International Journal of Biochemistry & Cell Biology

IF: 4

Published: 2005

Results: The AMA exhibits different specificities for N-acetyl lactose amide type and glycan oligosaccharide-mannoside type. The authors researched the action of this lectin on cells of the immune system. The inflammatory activity of the lectin was also researched using models of neutrophil migration in vivo, neutrophil chemotaxis in vitro, and macrophage culture. When applied to the peritoneal cavity of rats, AMA induced significant and dose-dependent neutrophilic leukocyte migration. Secondly, pretreatment with mercaptoacetate increased the number of peritoneal macrophages, thereby enhancing AMA-induced neutrophil migration. Reducing the number of peritoneal mast cells, however, did not alter AMA-induced neutrophil migration. Further experiments revealed that lectins induced neutrophil chemotaxis. In conclusion, all data suggested that the lectin had pro-inflammatory activity and induced neutrophil migration.

Applications

AMA is used to advance state-of-the-art technologies such as glycan arrays, differential expression analysis, and protein interaction analysis.
AMA is used in the development of coagulants.
AMA is used to explore the ability of lectins to bind exclusively to a particular carbohydrate.

Advantages

Our service team has accumulated a wealth of experience to support all of our client's requirements.
Our services include lectin extraction and testing to develop a total production program for our clients.

CD BioGlyco provides our clients with comprehensive lectin production services. We keep up with the trend of scientific and technological development, constantly update our knowledge and technology in the field of lectin, and do our best to help our clients with their research in lectin production. Please feel free to contact us for more detailed information.

References

Alencar, V.B.M.; et al. Pro-inflammatory effect of Arum maculatum lectin and role of resident cells. The International Journal of Biochemistry & Cell Biology. 2005, 37(9): 1805-1814.
Majumder, P.; et al. Insecticidal activity of Arum maculatum tuber lectin and its binding to the glycosylated insect gut receptors. J. Agric. Food Chem. 2005, 53(17): 6725-6729.

This service is for Research Use Only, not intended for any clinical use.