High-quality Services at CD BioGlyco

Lectins have good blood coagulation function, CD BioGlyco has long been committed to research on Lectin Production. We provide Natural Lectin Production services including but not limited to the production of Plant Lectins, Animal Lectins, and Fungal Lectins. In the field of plant lectin production, Astragalus mongholicus root (A. mongholicus root) lectin is an important lectin. CD BioGlyco provides a systematic A. mongholicus root lectin production service.

• Extraction and purification services

We obtain the crude product of A. mongholicus root lectin by cutting and grinding A. mongholicus root, adding Tis buffer for homogenization, centrifuging at high speed, adjusting pH, and concentrating the supernatant.

For purification, firstly we use ammonium salt to classify the precipitation, and then after freezing, centrifugation, pH adjustment, dialysis, and Affinity Chromatography separation to get the high-purity A. mongholicus root lectin.

• Property testing services
  • Relative molecular content
    Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Gel Filtration Chromatography methods are employed, and the common logarithm of the relative molecular mass of the standard proteins is used as the vertical coordinate, and the mobility is used as the horizontal coordinate for plotting. After the linear equation is derived, the relative molecular mass of the target protein is calculated based on its mobility.
  • Agglutination activity
    We add lectin samples to the microtiter plate. Next, it is diluted and added to rabbit erythrocyte suspension separately. After the oscillation operation, the coagulation effect is observed under the microscope.
    The lectin and cell suspension are mixed. After a series of operations such as standing, centrifugation, drying, cooling, etc., the difference in weight before and after is compared to determine the agglutination activity.
  • Binding activity
    Nucleophilic chromatography, gel permeation chromatography, and electrophoresis are used to test the subunit structure of A. mongholicus root lectin, and glycan binding. Currently, we find that A. mongholicus root lectin contains four subunits and has a high affinity mainly for α-D-mannose.

Fig.1 A. mongholicus root lectin production services. (CD BioGlyco)

Publication

Applications

• A. mongholicus root lectin has specificity and sensitivity and is used to specifically recognize glycan chains.
• A. mongholicus root is used in research on the modification and transformation of drug precursors.
• A. mongholicus root lectin is a glycoprotein that is used to probe glycan-protein interactions.

Advantages

• We produce A. mongholicus root lectin with high purity, good stability, and specific recognition.
• Our service team is sensitive and flexible to custom production solutions for our clients.
• Our production programs are quality-tested and fully meet the research requirements of our clients.

CD BioGlyco has rich experience in lectin production and is committed to providing clients with one-stop solutions for R&D, analysis, and testing. Our systematic service process helps our clients to shorten the lead time and to solve their problems in lectin research. Please feel free to contact us if you are interested in our services.