C. antennarius lectin is a sialic acid-binding lectin with high specificity, found in glycoproteins and gangliosides. Especially when bound to 9-O-acetyl and 4-O-acetyl sialic acid. The discovery of C. antennarius lectin enriches the types of sialic acid-binding lectins. Our professional scientific team provides clients with unique services from extraction, and purification to cell line culture.

C. antennarius Lectin Extraction Service at CD BioGlyco

• Raw material sources: We select C. antennarius of moderate size and weight for hemolymph extraction. For the obtained hemolymph, it is processed by low-temperature centrifugation before purification.

C. antennarius Lectin Purification Service at CD BioGlyco

At CD BioGlyco, we mainly offer agarose gel columns and high-performance liquid phase (HPLC) purification services. For the obtained hemolymph supernatant, dialysis, buffer soaking, and centrifugation followed by column purification. It is worth mentioning that the tube-by-tube collection is accompanied by the detection of absorbance. All elution conditions and process procedures are strictly controlled. We are committed to providing clients with high-purity purification services.

Fig.1 Flowchart of the purification of C. antennarius lectin. (CD BioGlyco)

C. antennarius Lectin Activity Analysis Service at CD BioGlyco

• Preparation service of anti-lectin we offer the service of determining the reactivity of antisera with purified lectin using an immunodot analysis. Using purified lectins and mixed with complete Freund's adjuvant, the rabbits are injected intramuscularly. Increase the amount of lectin injected over time. After a period of intensive injection, collection blood for determination.
• Lectin binding to erythrocytes assay service: Incubation with lectin, red cell suspension, and Tris-buffered saline bovine serum albumin (TBS-BSA) mixed at room temperature. Detection using sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE).
• Hemagglutination tests and Hemagglutination inhibition assay service: Both assays are performed in microtitre plates. Pay extra attention to the level of dilution, which greatly affects the results.

Applications of C. antennarius Lectin

• C. antennarius lectin is of great value in the localization and functional analysis of O-acetylsialic acids.
• C. antennarius lectin is used as a specific reagent for screening specific monoclonal antibodies.
• C. antennarius lectin could not only help identify the expression of O-acetylsialic acid-containing GD3 on the cell surface of the tumor but is also used for antibody specificity studies in the serum of disease patients.
• C. antennarius lectin is a serum factor required for efficient binding and uptake by phagocytes.

Advantages of Us

• Our professional and talented team has accumulated a lot of experience in Animal Lectin production and has been serving in the field of glycobiology for many years.
• Our company has precise equipment and specialized technicians to ensure the best quality of service.
• We offer a fully customized service according to the different needs of our clients.

CD BioGlyco offers comprehensive Lectin Production Services including Natural Lectin Production and Recombinant Lectin Production Services. We are often praised for our efficient service and high-quality products. Please feel free to contact us if you are interested in our services.