Enzyme-based GSL Purification Service

Enzyme-based GSL Purification Service

CD BioGlyco has specialized glycolipid purification technologies and we provide customized enzyme-based glycosphingolipid (GSL) purification services based on different substrates. We expect to share our extensive expertise and cutting-edge technology in the field of glycobiology.

Enzymatic Purification of GSLs

The principle of enzyme-based GSL purification is the hydrolysis of GSL using specific enzymes. Under specific conditions, the enzyme recognizes the structure and composition of the GSL molecule and breaks only specific chemical bonds during the hydrolysis process, thus breaking the GSL into units such as monosaccharides, disaccharides, or oligosaccharides. Different GSL molecules will produce different hydrolysis products under the action of different enzymes, and the purpose of purifying GSL can be achieved by separating or removing the different hydrolysis products during this process. The enzyme is specific, efficient, and selective in the purification process of GSL, which effectively improves the purification efficiency and purity.

Flow chart of enzyme-based GSL purification. Fig.1 Flow chart of enzyme-based GSL purification. (CD BioGlyco)

Enzyme-based Purification GSL Service

As a provider of GSL purification services, CD BioGlyco has a first-class biochemical laboratory and a professional GSL purification process. The services we provide include:

  • Phospholipase-based GSL purification service
  • This service is highly specific and can be applied to the purification of a wide range of GSL.

  • Lipase-based GSL purification service
  • This service targets fat-containing samples. The fatty esters in GSL are partially hydrolyzed by the esterification reaction of lipase for fatty esters. The GSL is then separated by a combination of ion exchange chromatography and high-performance liquid chromatography techniques.

  • Acid Enzyme-based GSL purification service
  • We utilize the properties of acidic enzymes to hydrolyze certain lipid impurities under highly acidic conditions to provide GSL with high purity.

  • Desaturase-based GSL purification service
  • This service separates saturated and unsaturated fatty acids and removes impurities containing unsaturated double bonds.


  • Crystallographic study of membrane proteins
  • Pathogen detection
  • GSL can be used as an anti-settling agent, viscosity adjuster, and drying agent in the coating industry

Advantages of Us

  • Mild conditions
  • High efficiency and high selectivity
  • High recovery rate

CD BioGlyco customizes enzyme-based GSL purification services to your specific needs, we making every effort to provide the best service to our customers. Moreover, we will perform validation procedures on the purification results to ensure the quality and purity of the products. If you are interested in our services, please contact us for more detailed information.


  1. Singh, A.K.; et al. Overview of fungal lipase: a review. Applied biochemistry and biotechnology. 2012, 166: 486-520.
This service is for Research Use Only, not intended for any clinical use.

About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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