Gene Knockout-based Cell Surface Glycoengineering Service
Gene Knockout-based Cell Surface Glycoengineering Service at CD BioGlyco
Cell surface glycoengineering technology based on gene knockout is widely used in the fields of biology and medical research, including but not limited to studying the mechanism of cell surface glycoproteins in tumor development, the mechanism of cell surface glycoproteins in immune regulation, the action mechanism of cell surface glycoproteins in neurobiology, the action mechanism of cell surface glycoproteins in pathogenic microbial infection, and the action mechanism of cell surface glycoproteins in stem cell biology.
Fig.1 Overview of glycoengineering strategies. (Narimatsu, et al., 2021)
With a focus on Cell Surface Glycoengineering, CD BioGlyco provides Technologies for Cell Surface Glycoengineering services that allow precise manipulation of the glycans, or sugar molecules displayed on the surfaces of living cells. Relying on the Glycoengineering Platform, we believe that we are the best partner for global clients. Our gene knockout-based cell surface glycoengineering services include but are not limited to:
Fig.2 Content of gene knockout-based cell surface glycoengineering service. (CD BioGlyco)
Publication
Published Data
Technology: Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells.
Journal: Metabolic Engineering
IF: 8.829
Published: 2019
Results: The authors used a CRISPR/cas9-mediated large deletion approach to knock out three sialidase genes (Neu1, 2, and 3) in the recombinant Chinese hamster ovary (rCHO) cell line that produces recombinant human erythropoietin. The results indicate that knockout of sialidase and pro-apoptotic genes in rCHO cells is a useful tool for generating large amounts of sialylated glycoproteins in fed-batch mode.
Here are some of the results shown in this article:
Fig.3 (A) Schematic illustration of the genomic target sites of sgRNAs in Neu1, 2, and 3. (B) The mRNA expression level of Neu1, 2, and 3 and (C) extracellular sialidase activity in culture supernatants in batch cultures of selected knockout clones. (Ha, et al., 2020)
Applications
- Cancer immunotherapy: By modifying the glycan structures on the surface of cancer cells, researchers make these cells more recognizable to the immune system. This enhances the immune system's ability to target and destroy cancer cells.
- Vaccine development: By manipulating the glycan structures on the surface of pathogenic microbes or viruses, researchers can create attenuated strains for vaccine development. This gene knockout-based approach enhances the immune response and improves the efficacy of vaccines.
- Stem cell therapies: Modifying the glycan profiles on the surface of stem cells improves their ability to integrate into host tissues and avoid immune rejection. This is particularly relevant in regenerative medicine and tissue engineering.
- Drug target identification: Gene knockout-based glycoengineering aids in the identification of cell surface glycoproteins that serve as drug targets. By selectively modifying the glycans on cell surfaces, researchers investigate how changes in glycan structures affect the function and interactions of specific proteins. This leads to the discovery of new drug targets and the development of targeted therapies.
Advantages
- We have a strong foundation in gene knockout technologies, enabling precise and efficient modifications to cell surface glycoengineering.
- Our team comprises a diverse set of experts from various scientific fields. This interdisciplinary approach means we bring different perspectives to problem-solving.
- We emphasize quality at every stage of the process. Rigorous quality control and validation procedures ensure that the gene knockout-based cell surface glycoengineering is consistent, reliable, and delivers robust results for your research.
At CD BioGlyco, the cutting-edge biotechnology service we provide encompasses a wide spectrum of innovative solutions aimed at harnessing the power of glycoscience. Our commitment to excellence is evident in every aspect of the Genetic Cell Surface Glycoengineering Service. Our team is dedicated to maintaining the highest standards and ensuring client satisfaction, particularly in the realm of gene knockout-based cell surface glycoengineering. If you are interested in our services, please do not hesitate to contact us for more details.
References
- Narimatsu, Y.; et al. Genetic glycoengineering in mammalian cells. Journal of Biological Chemistry. 2021, 296.
- Ha, T.K.; et al. Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures. Metabolic engineering. 2020, 57: 182-192.
