Principle of Glycogene Knockdown

Reducing the expression level of a target gene in a cell or organism by methods such as RNA interference (RNAi) results in suppression of its function. Since the effect of RNAi is usually to partially inhibit the expression of the target gene, it is referred to as gene knockdown. Aberrant glycogenes and glycan expression are closely associated with carcinogenesis, invasion, and metastasis of gastric cancer. For instance, silencing β-linked N-acetyl glucosamine transferase induces apoptosis in human cells through caspase-3 pathways. Therefore, searching for glycogene upstream regulators can effectively block the signaling pathway induced by aberrant expression of glycan genes and their glycans in cells. Glycogen knockdown helps researchers explore the biological function of glycosylation expression of specific genes, metabolic pathways, etc.

Fig.1 Cell line knockdown. (Pamudurti, et al., 2020)

Glycogene Knockdown Service at CD BioGlyco

At CD BioGlyco, we provide three gene knockdown methods based on a small interfering RNAs (siRNAs) vector, a short hairpin RNA (shRNA) vector, and a clustered regularly interspaced short palindromic repeats (CRISPR) vector. Different knockdown methods, vector types, tissue cell types, etc., will have an impact on the construction process. Glycogene knockdown service is not restricted by gene loci or cell types. It is realized in any gene, any locus, and all kinds of mammalian cells. At the same time, we client-specific design vectors and target gene interference sequences, according to the experimental needs of flexible control of vector expression. We combine with a variety of technical means of verification, to ensure the knockdown efficiency and accuracy. CD BioGlyco ultimately provides clients with high-quality and stable cell lines as well as comprehensive assay reports, including quantitative real-time PCR (qRT-PCR), western blot, mRNA level, protein level, and cell phenotype level.

Fig.2 Technical process of knockdown. (CD BioGlyco)

Applications

• Glycogene knockout is an excellent tool for limiting target gene expression.
• Glycogene knockout is suitable for high-throughput screening of drug molecules.
• Glycogene knockout is used as a model for the treatment of diseases caused by aberrant expression of sugar-binding chains and as an important biomarker providing a set of specific targets for therapeutic intervention.

Advantages of Us

• We have professional team support for you to solve the shRNA selection, vector design, experimental difficulties, and other problems.
• Glycogene knockout is 20-30 times more efficient than traditional.
• Our Glycogenomics Platform and shRNA database covers a wide range of species, allowing you to easily find the right shRNA to target your target gene.
• We provide customized conditional expression shRNA vectors, please send your request.

CD BioGlyco has many years of successful experience in the field of Glycogene Editing to help you conveniently select the target gene. Without the need for you to design your sequence, the whole process of customized special services for you. If you are interested in our service, please contact us further to obtain the specific service in detail.

References:

1. Qin, Y.; et al. MeCP2 regulated glycogenes contribute to proliferation and apoptosis of gastric cancer cells. Glycobiology, 2017, 27(4): 306-317.
2. Guo, R.; et al. Glycogenes mediate the invasive properties and chemosensitivity of human hepatocarcinoma cells. The international journal of biochemistry and cell biology. 2013, 45: 347-358.
3. Pamudurti, N.R.; et al. An in vivo strategy for knockdown of circular RNAs. Cell Discov. 2020, 6: 52.