CD BioGlyco provides serval HPLC-based analysis services for our client's requirement for LNB analysis. LNB is a component of human milk oligosaccharides (HMO) and one of the Bifidus growth factors. We have much experience and achievements in the field of HMO analysis, so we have the confidence to provide high-quality services to our clients.

Fig.1 The 2D structure of LNB. (PubChem)

Introduction

Human milk contains lacto-N-biose (LNB), also called lacto-N-biose I, which is a component of HMO and one of the Bifidus growth factors. Moreover, lacto-N-biocides could transform LNB into lacto-N-tetraose and lacto-N-neotetraose, the most prevalent constituents and key core structures of HMOs. Several cell types, such as the intestinal epithelium and blood group antigens, include LNB in the glycan domains of glycolipids and glycoproteins.

Fig.2 An in vitro synthetic enzymatic cascade pathway to produce LNB with ATP regeneration. (Du, et al., 2021)

Analysis Methods

The most used quantitative analytical technique for HMOs especially LNB measurement is high-performance liquid chromatography (HPLC). Based on HPLC, it may be combined with other different detection methods, including mass spectrometry (MS), fluorescence, evaporative light-scattering (ELS), pulsed amperometric detection (PAD), and ultraviolet (UV). The most highly developed method for measuring HMOs which does not require derivatization is high-performance anion-exchange chromatography (HPAEC) with PAD. HMOs should be derivatized to produce the detection signal or boost the detection sensitivity by HPLC analysis when utilizing UV or a fluorescence detector due to the poor UV absorption and lack of fluorescent characteristics. For the analysis of HMOs, NMR and capillary electrophoresis (CE) is also employed.

Fig.3 A chromatogram of a complex mixture (perfume water) obtained reversed-phase HPLC. (Wikipedia)

LNB Analysis Service at CD BioGlyco

CD BioGlyco has an outstanding and great analysis platform for LNB (or other samples) analysis, based on HPLC and other analysis instruments we have, we also combinate HPLC and PAD, UV, MS, and so on according to clients' requirements. Based on our powerful platform, We have the confidence to offer great help to clients in exploring the field of LNB analysis.

Advantages of Us

• We have an advanced and powerful analytics platform for LNB analysis.
• We have professional scientists and researchers for analysis services.
• We combinate HPLC with other technologies for better analysis results according to clients' needs.
• We also have a great service team to reply to client's questions in time and try our best to solve client's questions.

CD BioGlyco provides customized LNB analysis services for clients. If you are interested in our services, please feel free to contact us for more information. We welcome queries from all over the world, and our research experts will make every effort to assist our clients in discovering solutions to their concerns and meeting their requirements in the field of HMO analysis.

Reference:

1. Du, Z.; et al. Lacto-N-biose synthesis via a modular enzymatic cascade with ATP Regeneration. iScience. 2021, 24(3): 102236.