Lacto-N-biose (LNB) Production Service
Lacto-N-biose (LNB) has the value and potential for physiological function research and food market application. In this regard, CD BioGlyco accumulates many years of scientific research experience and provides you with high-quality services for LNB production.
The Existing Form of LNB
LNB is a disaccharide that plays an important role in the carbohydrate structure of several glycoconjugates. The compound exists in HMO, but cannot exist in the form of free disaccharides, and has acted as the structural unit of type I oligosaccharide with lacto-N-tetrasaccharide as the core structure. Oligosaccharides type I is only found in animals such as milk and monkeys, while oligosaccharides type II is found in mammalian milk. In addition, LNB glycans are present in the glycolipid and glycoprotein domains of several cell types, including intestinal epithelial cells and blood group antigens.
Fig.1 Lacto-N-biose I. (Kiyat, et al., 2020)
LNB as Natural Prebiotics
Probiotics are living microorganisms that have beneficial effects on host health at appropriate doses. Bifidobacterium, a genus of Gram-positive anaerobic bacteria, is a well-known probiotic and one of the major commensal microbiota in the gut of healthy humans and animals. Nondigestible food ingredients that promote probiotics' growth and/or activity are called prebiotics. Bifidobacteria predominated in the gut of breastfed infants, but not formula-fed infants, suggesting a prebiotic component of Bifidobacteria in breast milk. A large number of bifidofactors in breast milk consist of oligosaccharides. Human milk oligosaccharides are a good carbon source for the efficient growth of several strains of bifidobacteria. Studies have shown that LNB is a highly specific natural prebiotic for Bifidobacteria. It is the first time for newborn infants to obtain it from their mother's colostrum. It can be used by many Bifidobacterium strains, including 208 strains of 10 species and 4 subspecies.
Key Technologies
- Chemical synthesis: Chemical synthesis offers a robust approach for the production of LNB by using established organic chemistry techniques to build the disaccharide from monosaccharide precursors.
- Enzymatic synthesis: Enzymatic synthesis is a highly specific and environmentally friendly method for LNB production. This process utilizes natural enzymes to catalyze the glycosidic bond formation between galactose and N-acetylglucosamine.
CD BioGlyco's Lacto-N-biose (LNB) Production Service: A Comprehensive Guide
Our LNB production service is not just a synthesis offering; it's a partnership aimed at advancing your research and product development. We offer flexible solutions, from small-scale R&D samples to large-scale industrial quantities, and produce LNB and its various derivatives tailored to your specific needs. Our services are as follows:
- Initial Consultation and Project Design
After understanding your specific needs, including desired LNB purity, yield, and application, our team of experts will work with you to design a customized synthesis strategy that meets your project goals and timeline.
- Chemical Synthesis and Enzymatic Synthesis
Based on the project's design blueprint, we will implement chemical, enzymatic, or hybrid synthetic methodologies, followed by thorough optimization protocols to enhance yield and operational efficiency. Chemical synthesis offers precise stereochemical control, enabling the fabrication of tailored derivatives. This approach is highly scalable, making it ideal for large-volume production of LNB. Enzymatic synthesis typically incorporates in situ cofactor regeneration systems to boost reaction efficiency and reduce manufacturing expenses. Notable for its high substrate selectivity, this method mitigates undesired side reactions and streamlines downstream purification processes.
- Quality Control and Analysis
Each batch of LNB undergoes comprehensive quality control analysis, including high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy, to confirm compound identity, purity, and structural integrity.
- Downstream Processing and Purification
After synthesis, crude LNB undergoes a variety of chromatographic techniques to remove impurities and unwanted byproducts, ensuring the final product meets the stringent purity specifications required for your application.
- Final Delivery
The purified LNB product is packaged and delivered to you with a detailed Certificate of Analysis (CoA) and a comprehensive report. We ensure that our packaging and shipping solutions preserve the integrity of your product until it reaches your facility.
Workflow
Publication Data
Journal: Enzyme and Microbial Technology
IF: 3.257
Published: 2025
Results: This article explores engineering the GH136 lacto-N-biosidase LnbX from Bifidobacterium longum to boost its transglycosylation for synthesizing the HMO para-lacto-N-hexaose (pLNH) (using LNT as donor and lacto-N-neotetraose (LNnT) as acceptor). Fourteen LnbX mutants were designed via conservatively mutating active-site residues or shielding it with large hydrophobic residues; the top variant D416N raised pLNH yield 1.5-fold (to 27%). Optimizing reaction conditions (via central composite design) tripled the yield to a max 57% (100 mM LNT, 500 mM LNnT, 1 μM enzyme, 35°C, pH 6.5 after 3 h). LnbX D416N is stable under industrial pH/temp, uses LNFP-III as an alternative acceptor, and aids HMO diversification.
Fig.1 The model transglycosylation reaction catalyzed by LnbX and variants in the current work to produce pLNH from LNT and LNnT. (Vuillemin, et al., 2025)
Advantages
Guaranteed Quality and Purity
We guarantee a high-purity product, meticulously validated through stringent quality control measures.
Seamless Scalability from Research to Commercial
Our capabilities range from milligram-scale for early-stage research to multi-kilogram quantities for commercial applications, ensuring a seamless transition from lab to market.
Dedicated Custom Synthesis
We offer customized solutions to produce LNB derivatives, isotopically labeled LNB, or other specific modifications required for your unique research.
Applications
Functional Foods and Dietary Supplements
LNB can be used as a functional ingredient to develop various food products and dietary supplements to support digestive health and immune function in both children and adults.
Pharmaceutical R&D and Drug Targeting
LNB is a valuable tool in drug discovery and development, particularly for drugs targeting the gut microbiome, inflammatory bowel diseases, and other immune-related conditions.
Cosmetic and Skincare Formulations
LNB's moisturizing and protective properties make it a promising ingredient in cosmetic and skincare formulations.
Frequently Asked Questions
Customer Review
"The LNB batch we received was remarkably pure, exceeding the specifications. The consistency between the initial research sample and the subsequent kilogram-scale order has been flawless. We consider CD BioGlyco an indispensable partner."
- Dr. A.E., Senior Scientist
"Our research focuses on the selective growth of gut commensals, and the purity of the LNB substrate is critical to avoid confounding factors. The LNB from this service exhibited high purity, confirmed by our in-house NMR."
- P. V., Researcher at Glycoscience Center
"We needed a fast turnaround on a custom-labeled LNB derivative for a cell-tracing experiment. The team was highly communicative throughout the entire design and synthesis process. Their technical ability to execute the labeling chemistry while maintaining the delicate bond was superb."
- M.K., Director of Biotech Start-up
Associated Services
If you requires conjugating LNB to other molecule, our glycoconjugate synthesis service can provide a seamless solution.
Our advanced analytical services help you characterize LNB, its derivatives, and other glycans in your samples.
CD BioGlyco is your premier partner for high-quality LNB production. With our cutting-edge chemical and enzymatic synthesis technologies, commitment to quality, and a client-focused approach, we provide scalable and customizable solutions to drive your innovations. Please feel free to contact us to discuss your project, to request a quote, or to learn more about our services.
References
- Kiyat, W.E.; et al. Potential of lacto-N-biose I as a prebiotic for infant health: A review. Communications in Science and Technology. 2021, 6(1); 18-24. (Open Access)
- Vuillemin, M., et al. Enzymatic lacto-N-biose elongation of human milk oligosaccharides with the GH136 lacto-N-biosidase LnbX engineered for improved transglycosylation. Enzyme and Microbial Technology. 2025, 189: 110660.
