Mass spectrometry has been widely used in metabolomics and glycomics analysis. CD BioGlyco is equipped with advanced mass spectrometers, which provide customers with professional Mass Spectrometry-based analysis support in your research.

Profiling Strategies

"Glycomics" is an emerging field after genomics and proteomics. It is particularly important to develop rapid and sensitive methods to analyze abnormal glycoproteins related to diseases. Over the years, mass spectrometry (MS) has become a powerful tool for clarifying structures and attachment sites of glycans because of its outstanding 4S characteristics (sensitivity, specificity, stoichiometry, and speed).

There are two conventional glycoprotein analysis strategies based on mass spectrometry. One is the "top-down" MS-based strategy such as FT-ICR-MS and TOF-MS, that is, the complete glycoproteins are directly analyzed by MS and tandem MS without extensive separation or digestion. The other is the "bottom-up" MS-based strategy, which is most widely used in glycoprotein analysis. This strategy released glycans from glycoproteins by chemical or enzymatic methods followed by purification and analysis of carbohydrates and proteins respectively. In many cases, LC-ESI-MS has been used to analyze glycoproteins released from glycoproteins. In addition, the MALDI-MS method is also used because of its high-throughput measurement ability.

Fig.1 (I) Illustration of mass spectrometry imaging of N-linked glycans from FFPE mouse brain tissue. (II) MALDI-MS analysis of N-glycans released by PNGase F from the FFPE tissue section. (Banazadeh, 2017)

Services

CD BioGlyco has developed various mass spectrometry-based methods for glycan profiling. The technologies we provide include but are not limited to:

• Obtain O-glycoprotein sequence and O-glycosylation sites through matrix-assisted laser desorption ionization (MALDI) in-source decay (ISD) TOF/TOF-MS
• Analysis of glycosylation sites and glycan moieties of N-glycopeptide through a combination of high resolution/accurate mass and multiple dissociation techniques including CID, ETD, and HCD on the LTQ Orbitrap XL mass spectrometer
• Distinguish the isomeric glycans through ion mobility spectrometry (IMS) MS and nano-LC-Chip/TOF-MS.
• Comprehensively analysis of glycopeptides by C18-ESI-LC-MS/MS using both one and two-dimensional (1D and 2D) LC separations.

Applications

• Application in identifying abnormal glycans and glycoproteins in human serum to screen cancer biomarkers.
• Research on aberrant glycoproteins associated with diseases such as ovarian cancer and breast cancer.
• Application in biopharmaceuticals including antibody drugs.

Advantages of Us

• High-performance instruments
• Powerful analysis platform
• Experienced technicians in terms of MS experiment
• High-sensitive and high accuracy detection

Based on advanced spectral analysis instruments and extensive relevant experience, CD BioGlyco provides glycomic characterization service through a variety of mass spectrometry-based methods to meet customers' requirements in basic scientific research or clinical glycoproteomic analysis. If you are interested in our services, please contact us for more detailed information.

References:

1. Banazadeh, A.; et al. Recent advances in mass spectrometric analysis of glycoproteins. ELECTROPHORESIS. 2017, 38(1): 162-189.
2. Nishikaze, T.; et al. Differentiation of sialyl linkage isomers by one-pot sialic acid derivatization for mass spectrometry-based glycan profiling. Analytical Chemistry. 2017, 89(4):2353-2360.