N-glycan profiling has been widely used in the identification of various diseases, including cancer, Alzheimer's, and diabetes. CD BioGlyco has rich experience in glycan profiling. Our scientists use advanced lab equipment to process samples and generate results. CD BioGlyco has confidence to be your essential research assistant at every moment.
Protein post-translational modification (PTM) has been at the center of research for the worldwide scientific community. The most common PTM of protein is glycosylation, especially N-glycosylation. The synthesis of N-linked glycan starts from the endoplasmic reticulum and completes in the Golgi apparatus. N-glycosylation modification plays an important role in the process of protein folding and transportation. Its occurrence will lead to changes in stability, solubility and conformation of the protein. As glycoproteins, cytokines, monoclonal antibodies and other therapeutic proteins become popular in clinical research, there is an increasing demand for N-glycosylation analysis of these objects. However, the glycosylation analysis process is challenging due to the complexity and isomerism of glycans.
Fig 1. The biosynthesis of N-linked glycans present in the antibody (Q, Zhou.; H, Qiu. 2019)
At CD BioGlyco, we perform N-Glycan profiling use the following methods.
Hydrophilic interaction chromatography is a chromatographic technique used to improve the retention of strongly polar substances that are poorly retained in reversed-phase chromatography. InstantPC (ProZyme) and RapiFluor-MS (Waters) are used to label fluorophore to the glycosamine which produced after PNGase F digestion immediately and improve the sensitivity of the entire reaction. Before fluorescent labeling, detergents, non-volatile salts or substances with free amino groups which could interfere with the experiment should be removed.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is a new type of ionization biological mass spectrometry developed in recent years. It is suitable for high-throughput screening of proteins or other biological macromolecules and analyze the single nucleotide polymorphisms of oligonucleotides or genes. It is usually used as a powerful tool to analyze the molecular weight and molecular weight distribution of natural and synthetic polymers.
CD BioGlyco provides a full range of glycomics analysis services, including glycan analysis, site occupation, linkage analysis, glycan sequencing, etc, as well as many customized services. We use professionalism and efficiency to complete customer’s orders and save time and funds for customers while obtaining credible results.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.