Nucleoside Enzymatic Synthesis Service
Nucleic acid is an essential biomolecule in the process of life activities, and its basic structural unit is a nucleotide, which is composed of a pentose sugar, base, and phosphate. Which consists of base and pentose sugar and is also known as nucleoside. Nucleoside is a general term for a class of glycosides. Clinical studies have shown that nucleosides and drugs made from them are an important class of drugs for the treatment of viral infections, tumors, and AIDS. Of the antiviral drugs currently in use, 50% are nucleosides. Anti-tumor drugs such as cytarabine and trifluridine also belong to the nucleoside class. In recent years, nucleosides have become the focus of research by pharmaceutical workers.
In the synthesis of nucleosides, there are two main methods: Chemical Synthesis and biological (enzymatic) synthesis. These two synthesis methods have their advantages and have been widely considered and studied by researchers.
At CD BioGlyco, we leverage specialized enzymatic catalysis—specifically ribose phosphorylase for base-specific nucleoside conversion and deoxyribosyltransferase for purine/pyrimidine interconversions- to synthesize nucleosides efficiently under mild conditions, ensuring high specificity, minimal side reactions, and streamlined purification for antiviral and antitumor drug development.
To produce nucleosides more efficiently, CD BioGlyco has comprehensively researched biological methods to produce nucleosides. At the heart of this method is the catalytic action of enzymes. We offer two enzymes, ribose phosphorylase, and deoxyribosyltransferase, to catalyze the synthesis of nucleosides.
CD BioGlyco offers a method for the enzymatic production of nucleosides that is characterized by high efficiency, mild reaction conditions, simple operation, high reaction specificity, few side reactions, and simple isolation and purification of reaction products. In the process of nucleoside production, we will continue to optimize the production solution to provide customers with the best production service.
Fig.1 Uses of the two enzymes. (CD BioGlyco)
Combine the nucleobase, activated sugar derivative (often generated in-situ), appropriate enzyme(s), a suitable buffer (e.g., potassium phosphate), necessary cofactors, and water to the desired final volume.
Set and maintain the optimal temperature for enzyme activity. Determine the optimal reaction duration by monitoring progress over time. Ensure the mixture is consistently mixed (stirring or shaking).
Start the reaction, usually by adding the enzyme(s) last to the prepared mixture.
Periodically take small samples, immediately quench the enzymatic reaction (e.g., with acetonitrile or heat), and analyze them using analytical techniques like HPLC or TLC to track product formation and conversion rates.
Stop the reaction once optimal conversion is achieved, often by heat-inactivating the enzyme or precipitating components.
Separate the synthesized nucleoside monomer from unreacted materials, enzymes, and byproducts using methods such as chromatography (e.g., ion-exchange, reverse-phase) or precipitation.
Confirm the identity and purity of the isolated nucleoside monomer using analytical techniques like NMR spectroscopy, Mass Spectrometry, and UV-Vis spectroscopy.
DOI.: 10.3390/catal15030270
Journal: Catalysts
IF: 4.0
Published: 2025
Results: This review article explores advances in the enzymatic synthesis of nucleoside-5'-triphosphates (5'-NTPs) and their analogs. The authors critically compare enzymatic methods to traditional chemical synthesis, highlighting the superior regio- and stereoselectivity, milder conditions, and higher yields achievable with enzymatic cascades. They detail the key enzymes involved (phosphoribosyltransferases, nucleoside/NMP/NDP kinases, polyphosphate kinases - PPKs) and catalytic strategies for synthesizing both natural and modified 5'-NTPs starting from nucleobases or nucleosides. A major focus is placed on the critical role of efficient ATP regeneration systems (e.g., using PK, AcK, or PPKs) in driving these multi-step enzymatic reactions to high conversion. The authors conclude that enzymatic cascades, leveraging enzyme promiscuity and optimized cofactor regeneration, offer significant potential for efficient and sustainable production of these vital building blocks for applications in molecular biology, diagnostics, and mRNA therapeutics.
Building on the precision and sustainability of enzymatic synthesis for nucleoside monomers, our integrated production services deliver turnkey solutions for critical biomolecules:
CD BioGlyco has many years of experience in nucleoside production. We provide our clients with multiple routes to produce nucleosides and provide them with better service by continuously optimizing experimental solutions. If you are interested in our services, please feel free to contact us.
Reference
