O-glycan profiling is extremely important for biological macromolecules and cell research. CD BioGlyco has rich experience in glycan profiling. Our knowledgeable scientists use the most advanced experimental equipment to process samples and analyze data. CD BioGlyco has confidence to be your essential research assistant in the field of glycobiology.
Glycosylation is the process of adding carbohydrates to proteins or lipids under the control of enzymes. As a result of glycosylation, different proteins are marked with different labels, which change the conformation of peptides and increase the stability of proteins. Glycosylation can be categorized into 2 main types: N-linked and O-linked glycosylation. O-linked glycosylation is the transfer of carbohydrate chains to an oxygen atom of the hydroxyl group of serine, threonine, hydroxyproline or hydroxylysine. It is catalyzed by different glycosyltransferases and one monosaccharide is added each time. As with N-linked glycosylation, the last step is to add sialic acid residues. This reaction occurs in the trans face of the Golgi capsule and trans-Golgi network. In contrast to N-linked glycans, the release of O-linked glycans is a complex process due to the highly diverse nature of the O-glycan structure as well as the absence of specific enzymes that release the O-linked glycans attached to protein. Chemical release is the major method for O-linked glycans separation.
Fig 1. Schematic representation of structural elements in mucin type O-glycans (Li, Z.; Cai, W.G.2019)
At CD BioGlyco, we use the following methods to perform O-Glycan profiling.
Releasing glycans from glycoproteins for the purpose of further characterization is the most commonly used method for analysis of protein glycosylation. The only enzymatic method (O-glycanase) and several chemical methods (Reductive β-elimination/ Non-reductive β-elimination) were developed over the past decades for releasing O-glycans. Then multiple comprehensive strategies based on MS are used to analyze after releasing.
Fig 2. O-Glycan profiling flow chart - CD BioGlyco
CD BioGlyco provides a comprehensive and accurate O-glycan profiling as well as many customized services, the most advanced technology and well-trained researchers provide guarantee for your glycobiology research. We use professionalism and efficiency to complete customer’s orders and save time and funds for customers while obtaining credible results.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.