Glycosylation is an indispensable part of proteomics and a very critical modification of therapeutic proteins. CD BioGlyco has advanced technology and well-trained researchers to provide customers with systematic and rigorous scientific research services of monosaccharide quantitation, we have confidence to be your essential research assistant in the field of glycobiology.

Background

As specific glycosylation patterns are inherently linked to cellular physiology and environment, monitoring altered glycosylation levels during disease has become established as valuable means in the search for diagnostic and prognostic markers. Numerous studies also have shown that it can significantly regulate the biological activity, stability, and immunogenicity of vaccine, antibodies and many other key biomolecules and therapeutic proteins. Monosaccharide analysis is a regulatory requirement for the characterization of biopharmaceuticals in the ICH Q6B guidelines. These analyses are needed to determine the type of glycosylation (N-linked and O-linked) and the degree of glycosylation at all stages of drug development. For basic research and clinical purposes, more high-throughput and quantitative analysis methods have been developed to analyze monosaccharides. The instability of monosaccharide constituents necessitates precise sample handling, high-resolution analytical separation and sensitive detection techniques.

Fig 1. Graphical abstract of glycosylation research (Xiao, K.; et al. 2019)

Services

The developments of mass spectrometry have greatly accelerated the speed of glycomics research with improvements in its sensitivity and resolution. CD BioGlyco utilizes MS in combination with the variety of liquid phase separation techniques and fluorescence, and provides systematic methods to characterize, identify and quantify monosaccharides. The methods we provide include but are not limited to:

• MS Ionization: matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are the standard ionization techniques used, each with their own distinct advantages and disadvantages.
• Label Free Quantitation: it involves the MS analysis of glycans which release from their glycopeptides or proteins, in either their native state or after derivatization.
• Labelling Quantitation: labelling strategies have been designed to improve quantitative glycomics analysis by reducing the errors that instrument and sample matrix composition can induce in non-isotopic labelling quantitative analysis.

Applications

• Search for diagnostic and prognostic markers
• Proteins heterogeneity research
• Disease pathology and progression research
• Biopharmaceutical research

Advantages of Us

• Multiple types of markers
• Multiple technology platforms
• High sensitivity, selectivity and high throughput
• Cost-effective

CD BioGlyco provides services that combine a variety of glycobiology technology platforms to obtain systematic and accurate experimental data under the operation of well-trained researchers. We use professionalism and efficiency to complete customer's orders and save time and funds for customers.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Xiao, K.; et al. Mass spectrometry-based qualitative and quantitative N-glycomics: an update of 2017-2018. Analytica Chimica Acta. 2019.