CD BioGlyco provides customers with comprehensive and highly accurate polysaccharide quantitative analysis services. Our excellent technical team, technical platform and advanced data analysis software can guarantee our service quality. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Glycosylation plays a vital role in cell functions, including apoptosis, cell communication and pathogen invasion. The glycosylation pathways of mammalian proteins are complex. The diverse polysaccharide structures are attached to many different glycoproteins. As glycoproteins and glycolipids dominate the cell surface, polysaccharides constitute the first-level cellular mechanism on the cell surface, and can also affect the pathogenesis of diseases and serve as disease-specific markers.

To fully understand the functions of polysaccharides and glycoproteins, a thorough understanding of the total composition of polysaccharides present in a given sample is essential. Accurately comparing the relative quantification of glycoforms and oligosaccharides is a necessary step. The techniques for identifying and quantifying polysaccharide in a given sample are constantly improving, but as they evolve, data analysis becomes more complex. To solve this problem, many software for interpreting quantitative polysaccharide profiles have been developed, which can be used for biomarker discovery, glycoinformatics for genome and disease analysis, etc.

Services

Due to the complexity of polysaccharide, it is usually necessary to combine multiple technology platforms. Analytical techniques for quantifying polysaccharide include several orthogonal methods, such as high performance/ultra-performance liquid chromatography (H/UPLC), capillary electrophoresis (CE), mass spectrometry (MS), tandem mass spectrometry (MSn) and its combination. Based on our advanced technology, CD BioGlyco provides all-round services for polysaccharide quantification.

• Using size-exclusion chromatography (SEC) to study the molecular weight and structural characteristics of macromolecules. It can provide information about the entire molecular weight distribution. The separation can be carried out under specially designed conditions to maintain the stability and activity of the molecule while maintaining good resolution.
• Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) or pyrolysis direct chemical ionization-MS are in frequent use. By recording single ion spectra (Tandem-MS), structural information can be improved. Finally, the sequence of the polysaccharide chain can be reconstructed from the collected information.

Applications

• Discovery of biomarkers
• Genome and disease analysis research
• Cell communication mechanism research
• Pharmacology research

Advantages of Us

• Excellent technical team and systematic technical platform
• Combination of a variety of advanced data analysis software
• High specificity and efficiency
• Cost-effective

CD BioGlyco provides a variety of methods for the quantitative determination of polysaccharide, and has advanced data analysis strategies to get result. We use professionalism and efficiency to complete customer's orders and save time and funds for customers.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Walsh, I; et al. Quantitative profiling of glycans and glycopeptides: an informatics' perspective. Current Opinion in Structural Biology. 2016, 40: 70-80.