CD BioGlyco combines the first-class sample preparation strategies with a variety of high-precision experimental equipment to provide customers with high-quality polysialic acid quantification services. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Polysialic acids (polySia) are linear carbohydrate polymers which comprised of negatively-charged 5-N-acetylneuraminic acid (Sia, Neu5Ac) residues and connected by α-2,8-, α-2,9- glycosidic bonds or α-2,8 / α-2,9 alternating glycosidic bonds. In addition to polySia containing Neu5Ac units, other Sia units are also found in polySia polymers in nature: 5-N-hydroxymethylneuraminic acid (Neu5Gc) and deaminated- neuraminic acid (KDN). Polysialic acid regulates nerve cell development, nerve guidance and synapse formation by changing the adhesion of nerve adhesion molecules in the nervous system and also affects cell growth by interacting with cells and growth factors. The abnormal expression of polySia in cancer is highly correlated with disease progression. PolySia is also expressed on the surface of bacteria such as E. coli and is related to bacterial virulence. Therefore, quantifying the expression levels of polySia and polysialylated protein between normal and diseased tissues/cells is very important.

Fig 1. Structure of sialic acid and polysialic acid (Guo, X.; et al. 2019)

Services

Quantitative polysialic acid analysis in CD BioGlyco including but not limited to the following approaches.

• Fluorometric HPLC quantitative analysis: Fluorescence HPLC analysis has been proved to be very suitable for measuring the degree of polymerization of polySia. According to the final analyte, fluorescence quantitative analysis can be divided into two categories:
  • Direct method: quantifies polySia directly. HPAEC-FD, HPAEC-CAD
  • Indirect method: quantifies the derived hydrolysate by Fractionation-based RPLC-FD and Periodate fluorometric C7/C9 (sialic acid) analysis of polySia.
• Quantitative immunodetection: we provide antibodies to detect and quantify polySia and polysialylated proteins in biological matrices by enzyme-linked immunosorbent assay (ELISA), Western blotting and flow cytometry.

Applications

• Protein structure and function research
• Nervous system research
• Cell growth research
• Cancer and infectious diseases pathology and progression research

Advantages of Us

• Advanced sample preparation strategies
• Superior polymer separation and relative high tolerance to the complexity of biological samples
• High specificity and efficiency
• Cost-effective

CD BioGlyco provides a variety of methods for the quantitative determination of polysialic acid, and has advanced strategies to process samples at a high level. We use professionalism and efficiency to complete customer's orders and save time and funds for customers.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Guo, X.; et al. Recent advances in the analysis of polysialic acid from complex biological systems. Carbohydrate Polymers. 2019, 224: 115-145.