A mammalian cell expression system is a technology that uses mammalian cells as host cells to achieve protein expression by transfection to a foreign expression vector. At CD BioGlyco, we mainly provide expression services based on Chinese hamster ovary (CHO) cells and human embryonic kidney cells 293 (HEK293) because they are easy to process, grow fast, have extremely high transfection efficiency, and have highly efficient recombinant protein expression. In addition, we offer other cell line hosts including but not limited to:
Our company has a complete set of recombinant lectin production solutions and experienced operators. According to the experimental requirements and the characteristics of the target protein, suitable lactating cell lines, such as CHO cells and HEK293 cells, are selected to ensure the efficient expression of the target protein. Every step is tightly controlled.
Fig.1 The flowchart of recombinant lectin production service. (CD BioGlyco)
For purified recombinant lectins, we offer a wide range of analysis services.
Paper Title: Expression and characterization of recombinant chicken mannose binding lectin
Expression System: HeLa R19 cells
Journal: Immunobiology
Published: 2017
IF: 3.152
Results: HeLa R19 cells expressing recombinant chicken mannose binding lectin (RcMBL) were purified and examined. The results showed that the recombinant lectin was similar to the natural sources regarding structural functions and activity. The monomer mass was 26 kDa, corresponding to the predicted mass. The glycan microarray and bioactivity detection of RcMBL showed the strongest binding to high-mannose glycans. RcMBL as a valuable tool to study the role of molecular MBL between pathogens helpd to determine the role of MBLs in infections in chickens and other birds.
Fig.2 Plot of analyzed data for RcMBL. (Zhang, et al., 2017)
CD BioGlyco has a highly trained production team to serve our clients in the production of Recombinant Lectins. We offer a wide range of Expression Systems for clients to choose from and support both small and large-batch production services. The protein amount is changed according to the client's requirement, please feel free to contact us.
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About Us
CD BioGlyco is a leading biotechnology company specializing in glycobiology. We deliver high-quality products and services to support cutting-edge research worldwide.