Sialyllacto-N-tetraose a, sialyllacto-N-tetraose b, or sialyllacto-N-tetraose c (LSTa, LSTb, or LSTc) is an important complex oligosaccharide component in breast milk, which has many physiological effects on the healthy development of infants. CD BioGlyco provides clients with efficient production services for LSTa, LSTb, and LSTc, and hopes to support your research on complex oligosaccharides.

The Potentially Functional Food LSTa, LSTb, and LSTc

LSTa, LSTb, and LSTc are the dominant constituent of complex human milk oligosaccharides( HMOs). They effectively inhibit the adhesion of bacteria to epithelial surfaces, thereby blocking the first stage of the infection process, and are considered to play a major role in immune defense against viral infections and bacteria in the gastrointestinal tract of infants.

Fig.1 A chromatogram of a donkey milk sample. (Licitra, et al., 2019)

Some animal milk oligosaccharides are used as functional foods, such as replacing human milk as the bioactive ingredient in infant milk powder. Donkey milk has been considered to have this potential, studies have shown that donkey milk contains sialylated milk oligosaccharides such as LSTa, LSTb, and LSTc.

Synthetic Development of LST Derivatives

Many oligosaccharides are synthesized by classical chemical methods or only enzymatic methods. For the oligosaccharide structure with a clear regional and stereochemical layout and specific substitution mode, it is necessary to improve the efficiency of each step and try to simplify the purification step to improve the yield. In addition, using these two procedures to synthesize in synergetic mode is also a good method to obtain sialylated structures. One of the most suitable general methods for this situation is block synthesis. In recent years, some combination methods of chemical and chemoenzymatic synthesis for LST derivatives are reported.

LSTa, LSTb, and LSTc Production Service at CD BioGlyco

In addition to isolation and purification, CD BioGlyco provides a variety of methods to produce LSTa, LSTb, and LSTc. Our Production Strategy includes but is not limited to the following:

• Separation and purification technology
  Various high-grade standards and fully optimized separation routes.
• Combination of chemoenzymatic and enzymatic synthesis.
• Chemoenzymatic
  HMOs are prepared using a chemoenzymatic method as a starting material.
• Enzymatic synthesis
  Using a one-pot multi-enzyme system to synthesize products.
• Microbial Fermentation
  A complete and mature service product line from strain development to mass manufacturing.

Advantages of Us

• Combined application of production technologies.
• One-pot synthesis.
• Production in good yield.
• Continuous improvement and accumulation of technology.

CD BioGlyco has accumulated advanced production technologies for LSTa, LSTb, and LSTc. If you are interested in our services, please feel free to contact us.

Reference

1. Licitra, R.; et al. Profile and content of sialylated oligosaccharides in donkey milk at early lactation. LWT. 2019, 115: 108437.