Solanum tuberosum Lectin Production Service
Solanum tuberosum Lectin (STL) Production Service at CD BioGlyco
STL is a glycoprotein isolated from potatoes. It contains 50% carbohydrates that are composed of arabinose and galactose. It consists of two subunits (50 kDa). CD BioGlyco is a company that Produces Lectins for basic research in food and pharmaceuticals and is a large global supplier of Plant Lectin products. To increase lectin production, the first step is to increase the amount of crude lectin extracted from the starting material. This is followed by the critical purification procedure. We provide a one-stop STL production service.
Extraction and purification
We have abundant isolation methods to extract STL from Solanum tuberosum tubers. There are various types of lectin extraction methods, including traditional precipitation methods, ultrasonic extraction, microwave extraction and so on. We will develop a STL production program according to the client's requirements for lectins. Traditional precipitation methods and emerging technologies are used in combination.
Lectins have carbohydrate-binding specificity. We have developed a variety of purification methods to meet customer requirements for lectin purity, including Gel Chromatography, Affinity Chromatography, and others.
| Cat. No. | Product Name |
|---|---|
| XLC-68-01Q | STL, Unconjugate |
| XLC-68-03Q | STL, Alkaline Phosphatase Conjugate |
| XLC-68-04Q | STL, Biotin Conjugate |
| XLC-68-05Q-10 | STL, Colloidal Gold Conjugate, 10 nm |
| XLC-68-05Q-15 | STL, Colloidal Gold Conjugate, 15 nm |
| XLC-68-05Q-20 | STL, Colloidal Gold Conjugate, 20 nm |
| XLC-68-05Q-30 | STL, Colloidal Gold Conjugate, 30 nm |
Characterization
- Bradford assay: To determine the protein concentration of the extracts of the lectin.
- Haemagglutination assay: To detect the haemagglutination activity.
- SDS-PAGE, size-exclusion chromatography and MALDI-TOF-MS: To determine the molecular weight of the fractions.
Fig.1 Protocol for production of STL. (CD BioGlyco)
Publication
Technology: Ion exchange chromatography
Journal: Glycoconjugate Journal
IF: 2.926
Published: 2018
Results: In this study, a method was developed for the efficient extraction of heat-resistant lectins from potato tubers. The method involved ammonium sulfate precipitation, heat treatment, and ion exchange chromatography. The lectins extracted by the method were of high purity and the extraction time was greatly reduced.
Fig.2 SDS-PAGE analysis of the purified potato lectins at various steps. (Feng, et al., 2018)
Applications
- STL is used for Lectin Microarray. Lectin microarrays provide a fast and sensitive method for the analysis of glycans and glycoproteins. Researchers have used lectin microarrays to analyze the glycoforms and glycosylation properties of glycoproteins.
- STL is suitable for use in FLISA.
- STL is used to visualize the localization of specific saccharide moieties and the distribution of plasma membrane glycoproteins.
Advantages
- Extraction and purification from natural sources by chromatographic procedures produce a sizeable quantity of lectin.
- Combining new technology with traditional technology.
- The strict quality monitoring platform.
Over the years, through continuous experimentation and optimization, CD BioGlyco has a very comprehensive lectin production service. Our lectin production includes some of the most widely used lectins in cancer mechanism research, plant genetic engineering, tissue chemistry, biotechnology, and other areas of glycobiology. Please feel free to contact us if you need a one-stop STL production service. We are very much looking forward to providing you with high-quality lectin production services.
Reference:
- Feng, Y.; et al. A rapid and effective method for purification of a heat-resistant lectin from potato (Solanum tuberosum) tubers. Glycoconjugate Journal. 2018, 35: 403-409.
