GT3 serves as a precursor of more complex gangliosides belonging to the c-series. With CD BioGlyco's technology platform, your Lipidomics research, especially Sphingolipid Analysis, will be carried out by scientists with extensive experience in lipid analysis and characterization. We offer gas chromatography-mass spectrometry (GC-MS) and fast atom bombardment (FAB)-MS for trisialoganglioside 3 (GT3) analysis.

Structure and Functions of GT3

The structure of GT3 consists of a ceramide backbone linked to an oligosaccharide unit consisting of five sugar molecules. GT3 is elevated in the human retina, suggesting that it may play specific roles in the structure and function of the tissue. It also acts as a precursor to more complex gangliosides. In addition, GT3 levels are reduced in Alzheimer's disease models.

Fig.1 Gangliosides profiling by representative MS spectra. (Cavdarli, et al., 2020)

GT3 Analysis Service at CD BioGlyco

CD BioGlyco has provided reliable and cost-effective research Trisialoganglioside (GT) Analysis Services to clients all over the world and has received unanimous praise. We offer GC-MS and FAB-MS for GT3 analysis. The main analytical steps include the isolation and structural analysis of GT3. Taking human lung tissue samples as an example, our general analysis steps are as follows.

• Isolation of gangliosides

First, homogenize the lung tissue in a homogenizer. The lipid extract is then divided into two equal aliquots and perform a chromatograph. The column is eluted with chloroform, chloroform/methanol/water, chloroform/methanol/water, and chloroform/methanol/water, respectively. The last fraction contains the complex gangliosides, which are separated by anion exchange chromatography into monosialogangliosides, disialogangliosides, and GT. Individual gangliosides of the GT fraction are separated by thin-layer chromatography.

• Mass spectrometry analysis

Acidic glycosphingolipids are analyzed by MALDI-QIT-TOF mass spectrometer in negative ion mode. Samples are prepared by mixing acidic glycosphingolipid samples directly on the target with a 2'-4'-6' trihydroxy acetophenone monohydrate matrix solution containing a diammonium hydrogen citrate hydrate mixture.

• Structural analysis of GT3

Sialic acid is determined using the resorcinol assay.

Advantages of Us

• GC-MS provides enhanced sample identification, higher sensitivity, an increased range of analyzable samples, and faster results.
• Expert consultation on best practices for sample collection, pre-shipment processing, and storage to preserve sample integrity.
• State-of-the-art equipment and platforms.
• Different experimental programs for different requirements.

CD BioGlyco provides advanced Ganglioside Analysis Services according to the needs of clients. GC-MS and FAB-MS are trusted techniques for analyzing GT3. If you are interested in our services, please contact us for more details without any hesitation.

Reference

1. Cavdarli, S.; et al. Profiling of O-acetylated gangliosides expressed in neuroectoderm derived cells. International Journal of Molecular Sciences. 2020, 21(1): 370.