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Cell Surface Glycoprotein Glycoengineering Service

Significance of Glycoengineering

The mechanism of glycosylation in cells involves a complex metabolic network composed of auxiliary proteins and enzymes. Altering the glycosylation structure or modifying the existing pattern of cell surface glycoproteins will affect the interaction and function of glycoproteins, mainly including genetic engineering, enzyme engineering, and chemical synthesis. The engineering provides precise control of cell surface glycoproteins and makes them have specific recognition, adhesion, signal conduction, or drug interaction, and then plays a key role in biological research, disease treatment, and drug study.

Fig.1 Overview of glycoengineering strategies. (Narimatsu, et al., 2021)

One-stop Cell Surface Glycoprotein Glycoengineering Services at CD BioGlyco

CD BioGlyco has the world's leading glycoengineering and protein technology platform and professional scientific research personnel, who are fully capable of providing clients with one-stop glycoengineering services.

Glycosyltransferase engineering services

Specific glycosyl structures are introduced or changed on cell surface glycoproteins using glycosyltransferases, and the glycosylation modification models of glycoproteins may be adjusted, affecting their interaction properties, such as N-glycoprotein-based cell surface glycoengineering service and O-glycoprotein-based cell surface glycoengineering service.

Genetic engineering services

CD BioGlyco regulates the expression level of genes related to the glycosylation of cell surface glycoproteins by ways of gene editing, gene transfection, or gene knockout, thereby transforming the glycosylation state.

Chemical synthesis services

CD BioGlyco synthesizes sugar molecules with specific glycosyl structures and introduces them to the cell surface to achieve precise glycoengineering.

Analysis services

CD BioGlyco offers mature analysis and evaluation services-based cell surface glycoprotein glycosylation, including glycosylation site identification, glycosyl structure analysis, and glycoprotein interaction study, which help clients understand the glycosylation modification patterns and their impact on function and interaction.

Customized solutions

CD BioGlyco also provides personalized glycoengineering solutions according to the client's specific needs and research goals.
These services help clients achieve precise control and regulation of cell surface glycoproteins, thereby promoting research progress in related fields.

Lipid raft-based cell surface glycoengineering services. Fig.2 One-stop cell surface glycoprotein glycoengineering services. (CD BioGlyco)

N-glycoprotein-based Cell Surface Glycoengineering Service

CD BioGlyco provides mature N-glycoprotein-based cell surface glycoengineering services. By regulating the glycosyl structure and modification pattern of N-glycosylated proteins on the cell surface, the type, position, and quantity of glycosyl groups are changed, thereby precisely regulating their activity and interaction.

O-glycoprotein-based Cell Surface Glycoengineering Service

CD BioGlyco provides professional O-glycoprotein-based cell surface glycoengineering services. Protein glycosylation is a vital post-translational modification (PTM), containing O-linked glycosylation, which is the attachment of glycan units to the protein backbone through the side chains of Thr or Ser residues, without a consensus sequence.

Applications

Cell surface glycoprotein glycoengineering is applied in many fields.

Biomedicine: It is applied to study biological processes, for example, cell signal transduction, recognition, and adhesion processes.
Drug study: The interaction between drugs and cells may be adjusted to improve drug targeting, enhance drug efficacy, or reduce side effects. Moreover, new glycoprotein-targeted drugs or vaccines are developed by glycoengineering.

Highlights

CD BioGlyco has extensive experience in the field of cell surface glycoproteins and masters a variety of cell surface glycoprotein modification methods and technologies.
CD BioGlyco has advanced cell culture systems, analytical instruments, and related experimental equipment, enabling efficient cell surface glycoprotein glycoengineering services.
CD BioGlyco has established a complete quality management system to ensure the accuracy and reliability of cell surface glycoprotein modification results.

CD BioGlyco has accumulated rich experience and expertise in cell glycoengineering and integrated the most advanced technology to establish an efficient glycoengineering platform. Our platform provides high-level cell surface glycoprotein glycoengineering services with custom functions. Please feel free to contact us in time if you are interested in our services.

References

Natarajan, A.; et al. Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria. Nature Chemical Biology. 2020, 16(10): 1062-1070.
Narimatsu, Y.; et al. Genetic glycoengineering in mammalian cells. Journal of Biological Chemistry. 2021, 296.

This service is for Research Use Only, not intended for any clinical use.