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Cell Surface Sialic Acid Glycoengineering Service

Importance of Sialic Acid

Sialic acid is a type of α-keto acid sugar with a nine-carbon backbone, which is widely distributed in animal tissues. The sialic acid family includes various derivatives of the nine-carbon sugar neuraminic acid, which is applied as components of oligosaccharide chains of mucins, glycoproteins, and glycolipids, and they decorate the ends of sugar chains on the surface of cells or soluble proteins. The occurrence of many diseases is related to abnormal sialic acid modification on the cell surface. By performing sialic acid engineering services on the cell surface, these abnormalities are simulated or repaired to study the role of sialic acid in the pathogenesis of different diseases and explore treatments targeting sialic acid modifications.

Cell Surface Sialic Acid Glycoengineering Service at CD BioGlyco

CD BioGlyco provides professional cell surface sialic acid glycoengineering service, which is a technology that changes the characteristics and functions of cells by modifying the sialic acid glycosyl structure on the cell surface. We use chemical synthesis methods or enzyme-catalyzed reactions to directionally add sialic acid or specific types of sialic acid derivatives on the cell surface, which change the sialic acid modification level and structure of the cell surface to change its biological activity and interactions. We also adjust the structure of cell surface sialic acid, such as changing chain length, modification group type, etc., to achieve specific modifications of cell surface sialic acid. The main types of services provided are as follows.

N-Acetylneuraminic Acid (Neu5Ac)-based Cell Surface Glycoengineering Service

Neu5Ac is activated and pumped into the Golgi lumen as CMP-Neu5Ac through the antiporter, undergoes various post-synthetic modifications, and is then attached to the sugar chain through sialyltransferase to form different functional sialic acid complexes. CD BioGlyco provides professional Neu5Ac-based cell surface glycoengineering services.

N-Glycolylneuraminic Acid (Neu5Gc)-based Cell Surface Glycoengineering Service

Neu5Gc, one of the three basic forms of sialic acid, is a unique antigen that modifies cell surfaces in a variety of ways. CD BioGlyco provides proven Neu5Gc-based cell surface glycoengineering services.

2-Keto-3-deoxy-D-glycero-D-galacto-Nononic Acid (KDN)-based Cell Surface Glycoengineering Service

Under the catalysis of aialyltransferase, KDN monomers are attached to the ends of proteins, lipids, or oligosaccharides through α-glycosidic bonds to form KDN glycoconjugates, which are then transferred to the cytoplasm or cell membrane through vesicles. CD BioGlyco provide one-stop KDN-based cell surface glycoengineering services.

N-Glycolylneuraminic Acid (Neu4Ac)-based Cell Surface Glycoengineering Service

Neu4Ac is a neurocholine acid that belongs to the sialic acid class of compounds. It plays important physiological functions in organisms and is transferred to the cell surface through transport channels or carrier proteins. CD BioGlyco provides mature Neu4Ac-based cell surface glycoengineering services.

Deaminoneuraminic Acid (Kdo)-based Cell Surface Glycoengineering Service

Deaminoneuraminic acid (Kdo) is a compound with a similar structure to sialic acid and belongs to the sialic acid sugar class. It is transferred from the substrate to the bacterial outer membrane lipopolysaccharide (LPS) molecule via an enzymatic pathway and is ultimately localized to the bacterial cell surface. CD BioGlyco provides excellent Kdo-based cell surface glycoengineering services.

Acetylated Sialic Acids-based Cell Surface Glycoengineering Service

Acetylated sialic acids are products of acetylation modification on sialic acid molecules. Sialic acid is a common nine-carbon sugar acid found in animal and human cell surface sugar chains. Acetylated sialic acids are transferred to the cell surface through multiple enzymes and transporters. CD BioGlyco provides high-quality acetylated sialic acid cell surface glycoengineering services.

CD BioGlyco provides efficient sialic acid glycan analysis services. We use various analytical techniques, such as mass spectrometry, chromatography, and nuclear magnetic resonance, to conduct qualitative and quantitative analysis of modified or synthesized sialic acid glycans to evaluate the effects of sialic acid glycoengineering.

In addition, CD BioGlyco also provides the design and synthesis of sialic acid-specific probes. We provide sialic acid-specific probes with high selectivity and affinity for localization, detection, and visualization of sialic acid modifications on the cell surface.

Fig.1 Cell surface sialic acid glycoengineering services. (CD BioGlyco)

Applications

Immunotherapy: Sialic acid glycoengineering changes the structure of sialoglycos on the surface of immune cells and enhances or inhibits their interactions with other cells or molecules to regulate immune responses.
Vaccine development: Sialic acid on the cell surface is an important antigen for many pathogens. By mimicking or targeted modification of sialic acid, vaccine candidates are developed to stimulate specific immune responses and improve the immune system's ability to recognize and attack specific pathogens.
Drug screening: The importance of sialic acid in cellular function and interactions makes it a potential drug target. Through sialic acid glycoengineering services, drugs modified by sialic acid on the cell surface are screened, which provides new ways for disease treatment and drug development.

Highlights

CD BioGlyco has a professional technical team with in-depth research and rich experience in engineering modification and regulation of sialic acid sugars on the cell surface.
CD BioGlyco provides personalized cell surface sialic acid glycoengineering services based on client's needs, including the selection and design of different cell types, sialic acid types, and modification patterns.
CD BioGlyco ensures that the sialoglycomodified cells provided have high stability and consistency, and provide support for research and applications in different fields.

CD BioGlyco has established an advanced Cell Surface Glycoengineering Technology Platform. Our professional technical team will provide you with excellent cell surface sialic acid glycoengineering services. Please feel free to contact us if you would like to know more service details.

Reference

Natarajan, A.; et al. Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria. Nature Chemical Biology. 2020, 16(10): 1062-1070.

This service is for Research Use Only, not intended for any clinical use.