Deaminoneuraminic Acid (Kdo)-based Cell Surface Glycoengineering Service
Deaminoneuraminic Acid (Kdo)-based Cell Surface Glycoengineering Service at CD BioGlyco
CD BioGlyco has established a well-established Cell Surface Salivary Acid Glycoengineering Service. Kdo is found primarily in cell-surface podoplanar polysaccharides. Its biosynthesis is similar to that of Neu5Ac. In order to satisfy our clients' research needs for different salivary acids, we provide high-quality gene-editing services to target the introduction of Kdo. We also provide Kdo quantitative analysis service.
Fig.1 Types of Our Kdo-based cell surface glycoengineering service. (CD BioGlyco)
- Targeted introduction of Kdo
Based on reported biosynthetic pathways, we regulate Kdo synthesis by overexpression or knockout. Through Gene Editing, Kdo can be introduced in a targeted manner to enable specific modifications to cells. This strategy can be used for the study of Kdo bioactivity and its interaction with other substances
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Kdo quantitative analysis service
Kdo is a salivary acid derivative. Quantitative analysis of Kdo is important for the study of its function. We have a well-established Glycoengineering Platform. Based on our rich experience in salivary acid detection, we provide a one-stop Kdo quantitative analysis service for biological samples, glycoproteins, etc. Since Kdo exists at the end of the sugar chain of glycoconjugates. Its structure is complex and unstable. Generally, when analyzing and identifying Kdo, it needs to be released from the end of the sugar chain. We mainly use acid hydrolysis to separate it. After separation, Kdo needs to be chemically derivatized to make it into a stable structure. Among the many chemical derivatization methods, 1,2-diamino-4,5-methylenedioxybenzene (DMB) derivatization is the most classical. We mainly used DMB for the derivatization of Kdo. After derivatization, it is separated from the reaction mixture by reversed-phase (U) high-performance liquid chromatography ((U)HPLC). Finally, Kdo can be quantitatively analyzed by fluorescence detection.
Publication
Technology: (U)HPLC
Journal: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IF: 3
Published: 2023
Results: In this study, a chemical assay based on Kdo was developed. The endotoxin-specific marker Kdo was released by acid hydrolysis. Kdo was derivatized by reaction with DMB. The mixture was subsequently separated by (U)HPLC. Finally, the Kdo content of the sample is obtained by fluorescence detection. The endotoxin content can also be calculated from the Kdo content. This method developed in this study was validated to accurately determine the endotoxin content in the supernatant of gram-negative bacterial bioreactor samples.
Fig.2 Overlay of chromatograms of the Escherichia coli K12 cell culture R2 supernatant at harvest. (Hoffmann, et al., 2023)
Applications of Our Kdo-based Cell Surface Glycoengineering Service
- Kdo-based cell surface glycoengineering can be used for the development of methods for the detection of endotoxin and other levels in bacterial bioreactor samples.
- Kdo-based cell surface glycoengineering can be used for the study of Kdo-lipid interactions.
- Kdo-based cell surface glycoengineering can be used to study its synthesis in organisms.
Advantages of Us
- We have established a validated Kdo-based cell surface glycoengineering process. The methods used are highly targeted and provide accurate results.
- Based on our rich experience in quantitative analysis of salivary acids, we provide high-quality Kdo quantitative analysis services. A wide range of biological samples, glycoproteins, etc. can be analyzed.
CD BioGlyco keeps abreast of scientific developments and continuously improves its glycoengineering platform. We aim to provide high-quality, the most comprehensive, efficient and services to our clients. If you are interested in our Kdo-based cell surface glycoengineering services, please feel free to contact us for detailed experimental plans and prices. We are honored to serve you.
Reference
- Hoffmann, A.; et al. Application of a novel chemical assay for the quantification of endotoxins in bacterial bioreactor samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2023, 1228: 123839.
