CD BioGlyco always puts our clients' Plant Lectin Production needs first. SBA is a tetrameric glycoprotein that makes up 10% of soy protein. Each of its subunits is glycosylated. It specifically binds N-acetylgalactosamine and N-acetylgalactose due to the presence of two types of N-glycosidically linked carbohydrate units. Currently, soybean lectins are more widely used in immunology, food nutrition, and other fields. To meet the research needs of SBA in different fields, we provide a one-stop SBA production service.
Fig.1 Process of SBA production. (CD BioGlyco)
We mainly extract lectins from soybeans or roots. The crude extract of SBA is usually prepared by ammonium sulfate step-by-step precipitation. It is cumbersome, and ammonium sulfate will cause a small part of protein denaturation. Therefore, it is essential to find an efficient and safe method to prepare the crude extract of SBA for quantitative purification. We have improved the method by referring to the reports on the separation of SBA. The optimized preparation method shortens the experimental period. It is verified that the SBA yield is improved.
Based on the fact that SBA specifically and reversibly binds to the corresponding ligands or ligands, we further improve its purification method. At present, we mainly use Affinity Chromatography for the preparation of SBA. We also try to use new purification methods to shorten the purification steps and simplify the experimental operation.
We also provide comprehensive SBA analysis services. We use polyacrylamide gel electrophoresis (SDS-PAGE) to identify the subunit composition of SBA and its molecular weight. SBA is found to bind specifically to GalNAc, galactose, and LacNAc. We will also test its amino acid composition, sugar-binding specificity, etc.
With years of experience in Lectin Production and optimized production solutions, we offer a wide range of SBA products, including ferritin conjugate, peroxidase conjugate, etc.
| Cat. No. | Product Name | Cat. No. | Product Name |
|---|---|---|---|
| XGB1129 | SBA | XLC-28-05Q-20 | SBA, Colloidal Gold Conjugate, 20 nm |
| XGB1130 | SBA, Peroxidase Conjugate | XLC-28-05Q-30 | SBA, Colloidal Gold Conjugate, 30 nm |
| XLC-28-01Q | SBA, Unconjugate | XLC-28-08Q | SBA, Dylight 488 Conjugate |
| XLC-28-02Q | SBA, Agarose Conjugate | XLC-28-09Q | SBA, Dylight 549 Conjugate |
| XLC-28-03Q | SBA, Alkaline Phosphatase Conjugate | XLC-28-11Q | SBA, Ferritin Conjugate |
| XLC-28-04Q | SBA, Biotin Conjugate | XLC-28-12Q | SBA, FITC Conjugate |
| XLC-28-05Q-10 | SBA, Colloidal Gold Conjugate, 10 nm | XLC-28-13Q | SBA, Fluorescein Conjugate |
| XLC-28-05Q-15 | SBA, Colloidal Gold Conjugate, 15 nm | XLC-28-01Q-C | SBA, Unconjugate, Crude |
Technology: Capture and Release (CaRe)
Journal: Journal of Biological Chemistry
IF:4.8
Published: 2020
Results: In this study, a new method for the purification of lectin, called CaRe, was developed using SBA, etc. In this method, lectin was captured by a trapping agent (TCA). The captured lectin was released by competitive monovalent ligands. The purified lectin was later obtained by membrane filtration separation. The validation results of the separation of SBA, etc., showed that this method was accurate and simpler to operate than traditional protein purification methods such as ion exchange chromatography.
Fig.2 Different steps of the capture and release method. (Welch, et al., 2020)
CD BioGlyco continues to enrich lectin production services. We aim to provide our clients with comprehensive, high-quality lectin production services and a wide range of products. If you are interested in our SBA products or production and analysis services, please feel free to contact us for detailed plans and prices. We are very much looking forward to establishing a long-term cooperative relationship with you.
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