GSL Component Analysis by Lectin Assay
Glycosphingolipids (GSLs) play an important biological role in cells and are involved in many biological processes, including immunity and tumor metastasis, in addition to being involved in the development of a number of diseases. CD BioGlyco offers various customized GSL component analysis services by Lectin assay for our customers.
Introduction of Lectin Assay
Lectins are a class of proteins that are highly specific and able to bind to specific glycan molecules. By utilizing this property, Lectins strongly bond to fluorescein or enzymes to form fluorescein or enzyme-labeled Lectins. It is then added to the biological sample to be tested and the amount of sugar groups covalently bound to the Lectin in the sample is changed by fluorescence or enzymatic action. The results of the quantitative analysis then be obtained. In addition, Lectins can also isolate, enrich and purify glycan molecules by inducing coagulation and precipitation. Thus, Lectin assays are used as a common biochemical method for the quantification and detection of specific glycan molecules in protein or glycoprotein samples.
Our Service
CD BioGlyco has a cutting-edge, highly sensitive, and accurate solution for GSL component analysis, with particular experience in the extraction, purification, and component analysis of GSLs. The service we offer include:
- Identification of the composition of GSLs
- Determination of the relative content of different glycan structures in GSL systems
- Determining the spatial structure of GSLs
Different lectins bind to different types of GSLs, and we perform different Lectin tests on the samples to identify and characterize the different types of GSLs.
We performed lectin tests on samples using different specific lectins to determine whether the samples contained sphingolipids and to determine the amount of GSLs.
We provide services for the determination of the spatial structure of GSLs, such as intra-molecular rotation angle, spatial site resistance, and molecular conformation.
Fig.1 GSL component analysis services at CD BioGlyco. (CD BioGlyco)
Applications
- Research on the interaction between GSL and disease
- Application in cosmetics and health care products
- GSL function and structure analysis
Advantages of Us
- A variety of detection methods can be combined
- High sensitivity
- Professional researchers and reliable results
- Cost-effective service
As a provider of glycan analysis services, CD BioGlyco has cutting-edge technologies and professional researchers, and we strive to provide satisfactory and quality services to our clients. In addition, after the component analysis, we have a complete self-checking process to provide the most accurate analysis results.
If you are interested in our services, please contact us to obtain more information.
Reference
- Du, H.; et al. Analysis of glycosphingolipid glycans by lectin microarrays. Analytical chemistry. 2019, 91(16): 10663-10671.
