Despite their short chain lengths, the wide variety of positions and linkages of human milk oligosaccharides (HMOs) make their analysis challenging. After years of knowledge accumulation and research exploration, CD BioGlyco has developed a high-quality, fast, and accurate one-stop HMO Profiling solution.
HMOs are a group of naturally occurring oligosaccharides (OS) present in human milk. They are composed of five basic units, including an acidic monosaccharide, sialic acid (Sia); an amino sugar, N-acetylglucosamine (GlcNAc); and three monosaccharides, L-fucose (Fuc), D-galactose (Gal) and D-glucose (Glc). It has been reported that about 200 different HMOs have been characterized. LNT is the most abundant HMO in breast milk and is a representative of neutral HMO. It is a tetrasaccharide composed of Gal, GlcNAc, and lactose connected sequentially via β1-3 bonds, Galβ1-3GlcNAcβ1-3Galβ1-4Glc. Most HMOs are derived from sialylation or fucosylation based on lacto-N-triose (GlcNAcβ1-3Galβ1-4Glc) and LNT.
Fig.1 Chemical structure of LNT. (Wikipedia)
Nitrogen-containing LNT is considered one of the most important prebiotics in breast milk, supporting the growth of healthy bacteria in the gut microbiota, thereby promoting the development of the natural microbiota in newborns. LNT is one of the first functional foods that babies eat. LNT has been reported to be a non-competitive food source for Bifidobacterium infantis. Bifidobacteria not only cleave chemical bonds of LNT to digest OS in breast milk through specific transporters and glycosidases but also may inhibit the adhesion of harmful bacteria and the attachment of harmful bacteria proteins in the intestinal tract. In addition, LNT may also benefit the immune system in a supportive role.
High-performance anion-exchange chromatography/pulsed amperometric detection (HPAEC-PAD) is one of the mature techniques of HMO analysis that does not require derivatization. This technique is especially suitable for the analysis of neutral or acidic HMOs. At CD BioGlyco, we have developed high-sensitivity, reproducible, high-resolution, and high-accuracy HMO profiling solutions. We provide global clients with high-quality LNT analysis services in human milk samples through HPAEC-PAD.
In addition, we also provide diverse HMO products for global clients, including but not limited to:
| Cat. No. | Product Name | Cat. No. | Product Name |
|---|---|---|---|
| XCO0089Q | Lacto-N-biose | XCO0101Q | Disialosyl galactosyl globoside |
| XCO0090Q | N-Acetyl-D-lactosamine | XCO0102Q | Lacto-N-tetraose |
| XCO0091Q | 2'-Fucosyllactose | XCO0103Q | Lacto-N-neotetraose |
| XCO0092Q | 3-Fucosyllactose | XCO0104Q | Lacto-N-fucopentaose I |
| XCO0093Q | Lactodifucotetraose | XCO0105Q | Lacto-N-fucopentaose III |
| XCO0094Q | Lacto-N-triose I | XCO0106Q | Disialyllacto-N-tetraose |
| XCO0095Q | Lacto-N-triose II | XCO0107Q | 3'-Sialyllacto-N-neotetraose |
| XCO0096Q | 3'-Sialyllactose sodium salt | XCO0108Q | Sialyllacto-N-tetraose a |
| XCO0097Q | 3'-Sialyl-N-acetyllactosamine sodium salt | XCO0109Q | Sialyllacto-N-tetraose b |
| XCO0098Q | 6'-Sialyllactose sodium salt | XCO0110Q | Sialyllacto-N-tetraose c |
| XCO0099Q | 6'-α-Sialyl-N-acetyllactosamine sodium salt | XCO0111Q | p-Lacto-N-neohexaose |
| XCO0100Q | 3'-Sialyl-3-fucosyllactose |
CD BioGlyco is committed to providing comprehensive, reliable, and accurate analytical services for glycobiology research and development. Our service targets include but are not limited to universities, research institutes, and enterprises. If you are interested in our products and services, please feel free to contact us for more information.
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