Custom Deoxyribonucleic Acid (DNA) Synthesis Service
DNA is the fundamental storehouse of biological information in living organisms. It plays a key role in the development, growth, and functions of all known life forms. Since it was found, it has been an important investigative tool in many fields including chemical science and life science. For example, there have been significant advancements in synthetic genomics, particularly in the generation of viable microorganisms and other biological systems from synthetic genomes. Besides, a variety of DNA-based nanocarriers have been created that possess the internal features of DNA, including molecular recognition proficiency, structural flexibility, nanoscale controllability, biocompatibility, and biodegradability. These specially designated substances have been combined with many complex structural molecules, such as inorganic nanoparticles, biopolymers, and synthetic polymers to bestow functional properties onto nanocarriers. Therefore, a large number of DNAs are needed. Many technologies are developed to provide DNA in greater purity and quantity.
At CD BioGlyco, we utilize chemical synthesis and enzymatic synthesis as our core technologies. The chemical method employs solid-phase synthesis using DNA phosphoramidite nucleosides, following a cycle of deblocking, activation, capping, and oxidation to build oligonucleotides, followed by cleavage and deprotection. Enzymatic synthesis leverages terminal deoxynucleotidyl transferase (TdT) to assemble DNA under mild conditions, minimizing side products and damage, enabling the synthesis of longer strands. Additionally, the service offers synthesis of DNA hybrids by combining synthetic DNA with inorganic nanomaterials like gold, magnetic, or silver nanoparticles. These combined technologies enable the production of high-purity DNA and advanced hybrid materials for diverse research applications.
CD BioGlyco provides diverse methods for the custom synthesis of DNA, including chemical custom DNA synthesis and enzymatic custom DNA synthesis.
In our chemical method, we couple DNA phosphoramidite nucleosides to a solid support to construct an oligonucleotide chain. There is a four-step cycle that includes deblocking, activation, capping, and oxidation. That is used to attach each new nucleoside to the growing oligonucleotide. After formation, the entire polynucleotide is separated from the solid support. Deprotection and other post-synthesis processing are then performed.
Custom Enzymatic DNA Synthesis
Our enzymatic synthesis utilizes TDT as a DNA synthesis tool. The enzymatic method minimizes side product formation and DNA damage by utilizing enzyme specificity and mild conditions. As a result, longer DNA can be directly synthesized without compromising quality.
We also provide synthesis services for some DNA-hybrids that combine DNA with inorganic nanomaterials, including gold nanomaterial, magnetic nanoparticles, and silver nanomaterial. Those hybrids have potential as future material substitutes for some practical bio-applications. Those hybrids have made remarkable advancements in a wide range of cutting-edge applications, particularly in the field of biology. They arouse substantial interest from researchers.
DOI.: 10.1038/s41467-019-10258-1
Journal: Nature Communications
IF: 14.7
Published: 2019
Results: This work pioneers a terminator-free, template-independent enzymatic DNA synthesis platform leveraging TdT and apyrase under kinetic control. The authors optimized enzyme ratios and reaction conditions to enable iterative nucleotide additions, where TdT extends DNA initiators while apyrase degrades dNTPs to confine homopolymeric extensions to controlled lengths. Key innovations include characterizing nucleotide-specific polymerization kinetics (e.g., preferential extension of purine-terminated initiators), solid-phase synthesis on beads with cyclic dNTP delivery, and apyrase-mediated substrate degradation that replaces traditional blocking groups. The system's efficacy was validated by synthesizing DNA strands storing 144 bits of data ("hello world!") through sequential additions, with gel electrophoresis confirming stepwise length increases and revealing extension-length variations across nucleotide types. This approach eliminates the need for modified nucleotides or terminators, establishing a foundational enzymatic framework for scalable digital data storage in DNA.
Our DNA synthesis delivers sequence-specific DNA constructs for applications ranging from gene editing to diagnostic research. To ensure the highest fidelity and purity of these synthetic oligonucleotides, we provide integrated Nucleoside & Nucleotide Synthesis Services, which supply the essential molecular building blocks through diverse, precision-driven methods:
CD BioGlyco is an excellent company in the field of glycobiology, we happily offer tailored solutions to meet clients' specific requirements. Our company excels in diverse carbohydrate synthesis. Our team has leading academic authorities who have made significant contributions to the field. We also have deep knowledge and extensive experience to guarantee the highest level of expertise in custom DNA synthesis. If you are interested in our services, please do not hesitate to contact us.
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