Polynucleotide Is an Important Substance in Nature

Polynucleotide is a natural polymer that contains 13 or more nucleotide monomers. They are linked by covalent bonds. They form deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).

Key Technologies

At CD BioGlyco, our polynucleotide synthesis service leverages solid-phase phosphoramidite chemistry on automated synthesizers for precise DNA/RNA chain assembly, complemented by enzymatic methods using terminal deoxynucleotidyl transferase (TdT) for DNA and T7 RNA polymerase-based in vitro transcription for high-yield RNA synthesis, followed by rigorous PAGE and HPLC purification to deliver high-purity DNA/RNA for research.

Precision-Scaled, Glyco-Engineered: Synthesis Beyond Nucleotides.

CD BioGlyco offers a comprehensive range of techniques for the custom synthesis of DNA and RNA and provides diverse options including chemical custom synthesis and enzymatic custom synthesis.

Custom DNA Synthesis Service

Custom Chemical DNA Synthesis Service

• Our chemical approach for DNA synthesis couples DNA phosphoramidite nucleosides to a solid support and utilizes a four-step cycle of deblocking, activation, capping, and oxidation to construct the oligonucleotide chain.

Custom Enzymatic DNA Synthesis Service

• In our enzymatic synthesis, we use TdT as the primary enzyme for DNA synthesis.

Custom Hybrid DNA Synthesis Service

• In addition to our DNA synthesis services, we offer the synthesis of DNA hybrids incorporating various inorganic nanomaterials such as gold, magnetic nanoparticles, and silver.

Custom RNA Synthesis Service

• We offer a solid-phase chemical synthesis approach by using an automated synthesizer. In our method, the RNA chain is elongated step by step on a solid support. We efficiently and precisely synthesize RNA molecules with high purity and yield.
• Our in vitro transcription method uses a DNA template with a T7 RNA polymerase promoter. As a result, our method facilitates efficient synthesis of RNA molecules, leading to high yields usually in the milligram range.

DNA and RNA Purification Service

In addition, we provide DNA and RNA purification services to guarantee purified DNA and RNA for downstream research and applications. We effectively eliminate any unwanted by-products and impurities that may occur during the productive process by our technologies, including PAGE-based Purification Service and HPLC-based Purification Service.

Fig.1 The technologies of purification. (CD BioGlyco)

Workflow

Our polynucleotide synthesis workflow is designed for efficiency, quality, and scalability. Each step is carefully controlled and monitored to ensure the final product meets the highest standards.

• Project Consultation and Design

Our experts work with you to understand your project's specific needs, including sequence, length, scale, and modifications. We then recommend the optimal synthesis and purification strategy (chemical, enzymatic, or a hybrid approach) to achieve your goals.

• Synthesis

Based on the project design, the custom polynucleotide is synthesized. For chemical synthesis, this involves the automated, stepwise addition of phosphoramidite monomers. For enzymatic synthesis, it involves the controlled, enzyme-catalyzed addition of nucleotides to build the sequence.

• Deprotection and Cleavage

After synthesis is complete, the protecting groups are removed from the polynucleotide, and the newly synthesized strand is cleaved from the solid support.

• Purification

The crude synthesis mixture, which contains the full-length product along with truncated fragments and other impurities, is then purified using one of our advanced purification services (e.g., PAGE, HPLC, Cartridge-based). This critical step ensures the removal of contaminants and the delivery of a high-purity product.

• Quality Control and Final Delivery

Every synthesized and purified polynucleotide undergoes rigorous quality control, including mass spectrometry and capillary electrophoresis, to verify its sequence and purity.

Publication Data

Applications

• Interference RNA is applied for researching the functions of genes in cell culture and model organisms
• Recombinant DNA can be used to produce human insulin, eliminating the reliance on animal sources and enabling its widespread usage in the pharmaceutical industry.
• DNA technology is useful in agriculture. It enhances crop yield by transferring certain genes and increases nutrition value and shelf life by modifying certain genes.
• DNA can be used in RFLP and STR to identify suspects.

Advantages

• We utilize advanced polynucleotide synthesis techniques and optimal synthesis methods to ensure the accuracy and integrity of the structures.
• Our synthesis process undergoes rigorous quality control measures to provide reliable and consistent results.
• We have advanced PAGE and HPLC technologies to purity those polynucleotides we custom to ensure our quality of service.

Frequently Asked Questions

Associated Services

Our polynucleotide synthesis service delivers high-purity DNA/RNA sequences tailored to your length and modification requirements. To ensure these synthetic products meet application-specific purity standards, we provide specialized Oligonucleotide Fragment Purification Services, each optimized for critical impurity removal:

CD BioGlyco is a leader in the field of glycobiology, we are committed to offering carbohydrate synthesis services to meet your specific needs. We provide accurate and efficient synthesis solutions with our advanced techniques. With our simple processes and efficient project management, we guarantee the perfect completion of the service within the agreed time. Feel free to contact us as soon as possible if you are interested in our services.