Polynucleotide Synthesis Service
Polynucleotide Is an Important Substance in Nature
Polynucleotide is a natural polymer that contains 13 or more nucleotide monomers. They are linked by covalent bonds. They form deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
DNAs are the most common polynucleotides that are comprised of sugar deoxyriboses. There are two spiral chains in them. The structure contains diverse bases: adenine, guanine, thymine, and cytosine. They play a crucial role in storing and transmitting genetic information.
RNAs are the most basic polynucleotides in the biological system and possess the pentose sugar riboses. It is an unbranched polynucleotide formed by a single strand. The substance contains four nitrogenous bases, adenine, guanine, cytosine, and uracil. They are classified into several types, such as messenger RNA (mRNA) and transfer RNA (tRNA).
Key Technologies
At CD BioGlyco, our polynucleotide synthesis service leverages solid-phase phosphoramidite chemistry on automated synthesizers for precise DNA/RNA chain assembly, complemented by enzymatic methods using terminal deoxynucleotidyl transferase (TdT) for DNA and T7 RNA polymerase-based in vitro transcription for high-yield RNA synthesis, followed by rigorous PAGE and HPLC purification to deliver high-purity DNA/RNA for research.
Precision-Scaled, Glyco-Engineered: Synthesis Beyond Nucleotides.
CD BioGlyco offers a comprehensive range of techniques for the custom synthesis of DNA and RNA and provides diverse options including chemical custom synthesis and enzymatic custom synthesis.
Custom Chemical DNA Synthesis Service
- Our chemical approach for DNA synthesis couples DNA phosphoramidite nucleosides to a solid support and utilizes a four-step cycle of deblocking, activation, capping, and oxidation to construct the oligonucleotide chain.
Custom Enzymatic DNA Synthesis Service
- In our enzymatic synthesis, we use TdT as the primary enzyme for DNA synthesis.
Custom Hybrid DNA Synthesis Service
- In addition to our DNA synthesis services, we offer the synthesis of DNA hybrids incorporating various inorganic nanomaterials such as gold, magnetic nanoparticles, and silver.
- We offer a solid-phase chemical synthesis approach by using an automated synthesizer. In our method, the RNA chain is elongated step by step on a solid support. We efficiently and precisely synthesize RNA molecules with high purity and yield.
- Our in vitro transcription method uses a DNA template with a T7 RNA polymerase promoter. As a result, our method facilitates efficient synthesis of RNA molecules, leading to high yields usually in the milligram range.
DNA and RNA Purification Service
In addition, we provide DNA and RNA purification services to guarantee purified DNA and RNA for downstream research and applications. We effectively eliminate any unwanted by-products and impurities that may occur during the productive process by our technologies, including PAGE-based Purification Service and HPLC-based Purification Service.
Fig.1 The technologies of purification. (CD BioGlyco)
Workflow
Our polynucleotide synthesis workflow is designed for efficiency, quality, and scalability. Each step is carefully controlled and monitored to ensure the final product meets the highest standards.
- Project Consultation and Design
Our experts work with you to understand your project's specific needs, including sequence, length, scale, and modifications. We then recommend the optimal synthesis and purification strategy (chemical, enzymatic, or a hybrid approach) to achieve your goals.
- Synthesis
Based on the project design, the custom polynucleotide is synthesized. For chemical synthesis, this involves the automated, stepwise addition of phosphoramidite monomers. For enzymatic synthesis, it involves the controlled, enzyme-catalyzed addition of nucleotides to build the sequence.
- Deprotection and Cleavage
After synthesis is complete, the protecting groups are removed from the polynucleotide, and the newly synthesized strand is cleaved from the solid support.
- Purification
The crude synthesis mixture, which contains the full-length product along with truncated fragments and other impurities, is then purified using one of our advanced purification services (e.g., PAGE, HPLC, Cartridge-based). This critical step ensures the removal of contaminants and the delivery of a high-purity product.
- Quality Control and Final Delivery
Every synthesized and purified polynucleotide undergoes rigorous quality control, including mass spectrometry and capillary electrophoresis, to verify its sequence and purity.
Publication Data
DOI.: 10.3390/app12031543
Journal: Applied Sciences
Published: 2022
IF: 2.5
Results: Scrupulous comparative evaluation confronts dichotomous fabrication paradigms: synthetic chemical pathways (operative within abbreviated nucleotide sequences) and enzymatic in vitro transcription systems (preferable for extended polynucleotide architectures). Meticulous deconstruction follows regarding each technique's fundamental mechanisms, catalytic efficiencies, inherent constraints, and practical implementation logistics. Crucially, the investigation incorporates empirical observations derived from systematic experimentation quantifying yield optimization across diverse RNA molecular topologies. This taxonomic endeavor ultimately constructs an intellectual scaffold enabling researchers to implement determinative selection frameworks—balancing chemical precision against transcriptional scalability—when confronting particular ribonucleic acid targets intended for structural interrogation at industrial production thresholds.
Applications
- Interference RNA is applied for researching the functions of genes in cell culture and model organisms
- Recombinant DNA can be used to produce human insulin, eliminating the reliance on animal sources and enabling its widespread usage in the pharmaceutical industry.
- DNA technology is useful in agriculture. It enhances crop yield by transferring certain genes and increases nutrition value and shelf life by modifying certain genes.
- DNA can be used in RFLP and STR to identify suspects.
Advantages
- We utilize advanced polynucleotide synthesis techniques and optimal synthesis methods to ensure the accuracy and integrity of the structures.
- Our synthesis process undergoes rigorous quality control measures to provide reliable and consistent results.
- We have advanced PAGE and HPLC technologies to purity those polynucleotides we custom to ensure our quality of service.
Frequently Asked Questions
Associated Services
Our polynucleotide synthesis service delivers high-purity DNA/RNA sequences tailored to your length and modification requirements. To ensure these synthetic products meet application-specific purity standards, we provide specialized Oligonucleotide Fragment Purification Services, each optimized for critical impurity removal:
CD BioGlyco is a leader in the field of glycobiology, we are committed to offering carbohydrate synthesis services to meet your specific needs. We provide accurate and efficient synthesis solutions with our advanced techniques. With our simple processes and efficient project management, we guarantee the perfect completion of the service within the agreed time. Feel free to contact us as soon as possible if you are interested in our services.
Reference
- Ryczek, M.; et al. Overview of methods for large-scale RNA synthesis. Applied Sciences. 2022, 12(3): 1543. (Open Access)
