O-Fuc Pathway: A Unique Glycosylated Form

O-fucosylation (O-Fuc) is an important glycosylation modification in which fucose is covalently attached to a serine or threonine residue's hydroxyl group (O-linkage). Protein O-fucosyltransferases (POFUTs) are essential enzymes that catalyze the synthesis of protein O-Fuc. CD BioGlyco provides glyco-engineered cell construction services based on the two distinct O-Fuc pathways (POFUT1 and POFUT2 directed). The two O-Fuc pathways serve epidermal growth factor-like (EGF) repeat sequences (e.g. Notch) or thrombospondin EGF type 1 repeat sequences (TSRs, e.g. thrombospondin 1). Our staff has extensive experience in Glycan Display and Cell-based Glycan Display Array.

Unlocking the Potential of O-Fuc Pathway with Our Glycoengineering Cell Construction Service

• Glycosyltransferase-based genetic engineering service at CD BioGlyco
  • At CD BioGlyco, we provide differential regulation of and assessment services for O-Fuc glycosylation. Our operators have extensive experience in gene editing services to quickly and accurately help clients complete knockout and knockin.
  • We provide efficient, fast, and accurate glycosyltransferase gene knockout services through the genetic information of O-Fuc glycosyltransferase genes. Meanwhile, we introduce non-endogenously expressed glycan features by site-directed knockin integration of the glycosyltransferase gene. We provide O-Fuc binding and signaling regulation services by controlling the expression of Fringe genes.
  • We help clients to show the increase and loss of specific glycosylation features on specific cells (as in human embryonic kidney 293 (HEK293) cells) through highly efficient gene editing services.
  • We show the different glycoconjugates by controlling for O-Fuc pathway specificity. We analyze the relationship between the regulatory role of glycosyltransferase genes and function and structure.
• Modification service at CD BioGlyco

We offer two modification services including modification of EGF repeat sequences and modification of TSRs in cells to construct O-Fuc pathway-based glyco-engineering cells. After modification of the site, the glycans are specifically extended. In addition, we specifically recognize and modulate EGF repeats or TSRs by enzymatic modifications (POFUT1, the Fringe enzymes, TSR O-fucosyltransferase, and focus-specific 1,3-glycosyltransferase).

Fig.1 Schematic diagram of O-Fuc pathway-based glyco-engineered cell construction service. (CD BioGlyco)

Publication

Applications of O-Fuc Pathway-based Glyco-engineered Cell

• O-Fuc pathway-based glycoengineering cell plays a key role in the study of the mechanisms of uterine angiogenesis and vascular remodeling. It is a potential diagnostic and therapeutic target for uterine disease.
• O-Fuc pathway-based glycoengineering cell construction is an effective tool for the production of homogeneous carbohydrate chains.
• The O-Fuc modification of the O-focus base transferase 1 and EGF repeats is important in the Notch function.
• O-FucT-1 and the O-fucose modification of EGF repeats play an important role in Notch function.
• O-Fuc pathway-based glycoengineering cell participates in the production of O-fucose-modified polypeptides.

CD BioGlyco helps clients reveal the genetic, biosynthetic, and structural correlations of glycan binding in their natural environments. Our operations team helps clients avoid some common problems. Please feel free to contact us for more information.