Lens Culinaris Lectin Production Service
Lens culinaris Lectin (LCA) Production Service at CD BioGlyco
CD BioGlyco customizes the perfect Lectin Production Service for different lectins. LCA is one of the more widely studied plant lectins. It consists of four subunits and is carbohydrate-specific for α-D-glucose and α-D-mannose. The LCA used in the current study is isolated from L. culinaris seeds and purified by Affinity Chromatography. LCA is found to agglutinate human erythrocytes but was not blood group specific. It has been tested that LCA inhibits viral infection and induces apoptosis of cancer cells to a certain extent, which is promising for application. As a leader in the field of Plant Lectin Production, we offer a wide range of LCA and its conjugate products and a one-stop LCA production service.
Fig.1 Process of LCA production. (CD BioGlyco)
- LCA production service
We obtain lectins from L. culinaris isolation. The seeds are first soaked in Tris-HCl buffer. After that, the seeds are stirred well and centrifuged to obtain the crude extract. Purification is one of the key steps to obtaining lectins. We used Ion Exchange Chromatography, affinity chromatography, and size-exclusion chromatography to purify the product. Finally, the purified LCA powder is obtained by dialysis drying.
L. culinaris species are abundant. The content and biochemical properties of the lectins extracted from different species of L. culinaris may vary. Upon request, we also test the biochemical properties of the extracted lectins, including molecular weight, hemagglutination activity, carbohydrate specificity, mass spectrometry, amino acid sequence, etc.
- LCA products
We have a wide range of LCA and its conjugates. The hemagglutination activity and agglutination titer of the products are measured. Our goal is to provide our clients with the best quality lectin products that are safe to use in research.
| Cat. No. | Product Name | Cat. No. | Product Name |
|---|---|---|---|
| XLC-41-01Q | LCA, Unconjugate | XLC-41-12Q | LCA, FITC Conjugate |
| XLC-41-03Q | LCA, Alkaline Phosphatase Conjugate | XLC-41-13Q | LCA, Fluorescein Conjugate |
| XLC-41-04Q | LCA, Biotin Conjugate | XLC-41-01Q-A | LCA-A, Unconjugate |
| XLC-41-05Q-10 | LCA, Colloidal Gold Conjugate, 10 nm | XLC-41-01Q-B | LCA-B, Unconjugate |
| XLC-41-05Q-15 | LCA, Colloidal Gold Conjugate, 15 nm | XLC-41-02Q-A | LCA-A, Agarose Conjugate |
| XLC-41-05Q-20 | LCA, Colloidal Gold Conjugate, 20 nm | XLC-41-02Q-B | LCA-B, Agarose Conjugate |
| XLC-41-05Q-30 | LCA, Colloidal Gold Conjugate, 30 nm | XLC-41-14Q | LCA, Peroxidase Conjugate |
| XLC-41-05Q-40 | LCA, Colloidal Gold Conjugate, 40 nm | XLC-41-15Q | LCA, Rhodamine Conjugate |
| XLC-41-05Q-5 | LCA, Colloidal Gold Conjugate, 5 nm | XLC-41-16Q | LCA, Cy3 Conjugate |
| XLC-41-11Q | LCA, Ferritin Conjugate | XLC-41-17Q | LCA, Cy5 Conjugate |
Publication
Technology: Ion Exchange Chromatography, Affinity Chromatography, Size Exclusion Chromatography
Journal: Chinese Medicine
IF: 2.96
Published: 2015
Results: In this study, ion exchange chromatography, size-exclusion chromatography, and affinity chromatography were used to purify the lectin isolated from green spotted lentil seeds (L. culinaris) (GSLL). The purification scheme was tested to be more effective with a purification rate of about 50-fold. The extracted lectins were characterized. Its biochemical characterization showed that GSLL consists of two subunits (17 and ~4 kDa) with a size of 21 kDa. Addition of mannose and glucose decreased its hemagglutination activity. Rhamnose, galactose, arabinose, etc., had no effect on its hemagglutination activity. This suggested that GSLL was a mannose- and glucose-specific lectin.
Fig.2 Elution profile for purification of GSLL through (a) Mono Q and (b) Superdex 75. (Chan, et al., 2015)
Applications of LCA Production
- LCA is used to assay its antiviral activity and thus provide data to support antiviral strategies.
- The inhibitory carbohydrates of LCA are α-Methylmannoside and α-Methylglucoside. Based on it, our LCA products of high purity are used in affinity chromatography to isolate glycoconjugates.
- Based on the sugar-binding specificity of LCA, our LCA products are used to recognize specific carbohydrate structures.
Advantages of Us
- We are constantly improving our technology for the production of lectins and lectin conjugates. We aim to fulfill our client's research needs in different fields.
- We have a wide range of LCA and its conjugate products, including biotin conjugate, colloidal gold conjugate, etc.
- We are committed to innovation, rigor, and quality and provide our clients with outstanding lectin production solutions.
CD BioGlyco is determined to provide first-class lectin products for clients. Relying on our excellent production technology and good service guarantee, we have won wide praise from our clients. Please feel free to contact us if you have any questions or concerns about our products or production services.
Reference
- Chan, Y. S.; et al. Lectin from green speckled lentil seeds (Lens culinaris) triggered apoptosis in nasopharyngeal carcinoma cell lines. Chinese Medicine. 2015, 10(1): 1-9.
