The Roles of Oligonucleotide Fluorophore Modification

Oligonucleotides play an important role in a variety of critical analyses after being labeled with many fluorescent dyes in different forms. Fluorescent labeling is accomplished by inserting one or more fluorescent dyes directly into the synthesized sequence. Alternatively, oligonucleotides with reactive functional groups are linked to reactive groups on the fluorescent dye molecule by artificial labeling to complete the labeling. The fluorescent groups attached to the 5' end, the 3' end, the base end of the nucleoside or Phosphorylation, and the structure of the ribose using covalent bonding. Upon completion of labeling, the fluorescently labeled product needs to be purified for further use. The indispensable position in many biotechnologies makes fluorescently labeled oligonucleotide probes play a great role in disease prevention and diagnosis, food safety testing, ecological and environmental testing, forensic evidence identification life science research, etc.

Fig.1 Schematic diagram of single-labeled fluorescent oligonucleotide probe detection. (Zhu, et al., 2019)

Oligonucleotide Fluorophore Modification Service at CD BioGlyco

CD BioGlyco provides different fluorophore modification services including Fluorescein, Carboxyfluorescein (FAM), Tetrachlorofluorescein (TET), Hexachlorofluorescein (HEX), Cy3&Cy5, TAMRA, Dabcyl and BHQ labeling service. Various fluorescent labeling processes and products are optimized to ensure the best parameters.

• We have advanced solid-phase synthesizers and other large-scale instrument platforms. From synthesis and purification to fluorescence modification, and even to the activity control experiments professionally customized for you to provide high yields and high purity of fluorescence modification products.
• For product purification, you choose a variety of purification methods, such as high-performance liquid chromatography (HPLC), ion exchange column, gel electrophoresis, and other methods, using different methods to achieve the purpose of product enrichment. Among them, liquid chromatography mainly includes anion-exchange chromatography and the reverse ion-pair method, which has a high resolution, high purification efficiency, and can be automatically purified and prepared on a large scale.
• According to different experimental needs, we provide comprehensive qualitative and quantitative monitoring and analytical methods. Through the fluorescent group sequence comparison and spectroscopic determination, to achieve high sensitivity and specificity detection.

Fig.2 Classification of different fluorophore labeling. (CD BioGlyco)

Applications of Oligonucleotide Fluorophore Modification

• Oligonucleotide fluorophore modification can be used in gene chip technology.
• Oligonucleotide fluorophore modification is applied for the detection of multiple hybridization reactions.
• Oligonucleotide fluorophore modification is suited for real-time fluorescence PCR assay.

Advantage

• Based on different labeling reagents, we offer our clients a variety of efficient modification services.
• Our professional team provides clients with high-level oligonucleotide fluorophore modification services.

CD BioGlyco provides high-quality Oligonucleotide Modification services to clients all over the world. We are capable of providing efficient and reliable services to accelerate our clients' scientific research by shortening the project time. If you are interested in our service, please feel free to contact us further to introduce the specific service in detail.