Oligonucleotide Modification Service

The Exciting Potential of Oligonucleotide Modification

Oligonucleotide modification involves precise adjustments to nucleic acid structure, including sugar backbone, bases, linkers, and fluorophores. These changes augment stability, binding, and specificity, broadening applications across disciplines. Modified oligonucleotides are crucial in gene therapy, modulating expression, rectifying mutations, and targeting pathogenic sequences. Diagnostic use leverages them for precise genetic variants and pathogen identification. Additionally, these modified strands play pivotal roles in RNA interference, gene silencing, and aptamer-mediated drug delivery. Bridging fundamental research with practicality, oligonucleotide modification propels progress in personalized medicine, molecular biology, and biotechnology, fostering innovation across fields.

Fig.1 Common chemical modifications in antisense oligonucleotides. (Khan, et al., 2021)

Oligonucleotide Modification Service at CD BioGlyco

Based on oligonucleotide structure, CD BioGlyco has developed several oligonucleotide-based modification services for clients to study the optional application of oligonucleotide analogs. Besides oligonucleotide-based modification services, CD BioGlyco also offers Custom Oligonucleotide Synthesis Services and Nucleoside & Nucleotide Modification Services for the generation of a better custom research proposal for our clients.

Oligonucleotide-based Phosphorylation Modification Service

Modification of oligonucleotide phosphate groups to alter their properties and functions.

Oligonucleotide-based Phosphorothioate Backbone Modification Service

Oligonucleotide phosphorothioate modification replaces non-bridging oxygen with sulfur, enhancing stability and binding affinity.

Oligonucleotide-based Sugar Ring Modification Service

Oligonucleotide sugar ring modifications alter deoxyribose/ribose, improving stability, selectivity, and pharmacokinetics.

Oligonucleotide-based Bases Modification Service

Oligonucleotide base modifications alter nucleotide structure, enhancing binding specificity, stability, and function for applications including gene silencing and diagnostics.

Oligonucleotide-based Linker Modification Service

Oligonucleotide linker modification changes connecting segments, influencing spatial arrangement and interactions.

Oligonucleotide Fluorophore Modification Service

Oligonucleotide fluorophore modification incorporates fluorescent molecules, enabling visualization and tracking of DNA/RNA, pivotal in imaging and molecular studies.

Applications of Oligonucleotide Modification Service

Modified oligonucleotides serve as probes for detecting genetic variations, pathogens, and disease markers in techniques like PCR, qPCR, and DNA microarrays.
Modified siRNAs and miRNAs silence genes at the post-transcriptional level, advancing therapeutics for cancer, viral infections, and neurodegenerative diseases.
Fluorophore-labeled oligonucleotides aid in visualizing and tracking nucleic acids, proteins, and cellular processes using techniques like FISH and live-cell imaging.
Modified RNA sequences in mRNA vaccines enable targeted protein production, revolutionizing rapid vaccine development and distribution.
Modified oligonucleotides are pivotal for techniques like RNA-seq and ChIP-seq, enabling detailed analysis of gene expression and epigenetic modifications.

Highlights of Our Oligonucleotide Modification Service

CD BioGlyco built a well-equipment lab for our synthesis platform, which provides high-quality outcomes for our clients.
CD BioGlyco lab team exhibits strong attention to detail and follows rigorous quality control measures, ensuring the accuracy and precision of their experimental procedures and analysis.

CD BioGlyco provides custom oligonucleotide modification services for our clients, if you have any questions or require details information, please feel free to contact us.

References

Khan, P.; et al. RNA-based therapies: A cog in the wheel of lung cancer defense. Molecular cancer. 2021, 20: 1-24.
Seok S-H. Structural insights into protein regulation by phosphorylation and substrate recognition of protein kinases/phosphatases. Life. 2021, 11(9): 957.
Tripp R.A.; et al. The G glycoprotein of respiratory syncytial virus depresses respiratory rates through the CX3C motif and substance P. Journal of Virology. 2003, 77(11): 6580-4.
Sakurai, Y.; et al. Synthesis and properties of nucleobase-sugar dual modified nucleic acids: 2'-OMe-RNA and scpBNA bearing a 5-hydroxycytosine nucleobase. The Journal of Organic Chemistry. 2022, 88(1): 154-162.
Roberts, T.C.; et al. Advances in oligonucleotide drug delivery. Nature Reviews Drug Discovery. 2020, 19(10): 673-694.
Rublack, N.; et al. Synthesis of specifically modified oligonucleotides for application in structural and functional analysis of RNA. Journal of nucleic acids, 2011.
Saneyoshi, H.; et al. Thiol-responsive pro-fluorophore labeling: Synthesis of a pro-fluorescent labeled oligonucleotide for monitoring cellular uptake. Bioorganic & Medicinal Chemistry Letters. 2020, 30(13): 127222.

This service is for Research Use Only, not intended for any clinical use.