The Features of Oligonucleotide FAM Labeling

With the continuous screening of aptamers and the synthesis of more and more fluorescent moieties, the application of nucleic acid fluorescent probes constructed using aptamers combined with fluorescent moieties is becoming more and more widespread. FAM is a type of fluorescein derivative; it reacts quickly with amino groups and the products are more stable for the 488 nm spectral line of the argon-ion laser. FAM has the general properties of fluorescein derivatives and is stable in water. The recognition group is the key to the nucleic acid fluorescent probe. When the recognition group and the target respond, the nucleic acid fluorescent probe structure or conformation changes, producing the corresponding signal. Through the fluorescent signal changes to achieve the detection of the target, FAM-labeled Oligonucleotide Modification has been successfully applied in various industries.

Fig.1 Schematic representation of the fluorescent probe detection with FAM labeling. (Liu, et al., 2022)

FAM Labeling Service at CD BioGlyco

CD BioGlyco has accumulated countless experiences in FAM-labeled synthesis and purification. We provide a comprehensive range of carboxylic acid fluorescein modification services. We have always been well received by customers with quality service and reliable products.

• We are a professional biotechnology company with rich experience in DNA synthesis, gel electrophoresis, and HPLC in the synthesis and isolation of modified oligonucleotides.
• Our talent team has a strong knowledge base in NMR (¹H, ¹³C, ¹⁹F, ³¹P), Ultraviolet (UV), fluorescence, and mass spectrometry (MALDI-TOF and ESI-MS) in the characterization of compounds and oligonucleotide concatenations. We are capable of providing efficient and reliable services to accelerate our clients' scientific research by shortening the project time.
• For FAM-labeled oligonucleotides, we provide purification services. Equipped with a large instrumental analysis platform and perfect system activity laboratory to meet the needs of customers in various aspects.

Fig.2 Schematic diagram of analyzing and testing. (CD BioGlyco)

Applications of FAM Labeling

• FAM-labeled oligonucleotides can be used as a tool to detect DNA and miRNA biomarkers in clinical samples.
• FAM labeling is used in the detection of metal ions, small molecules, RNA, DNA, and substances.
• FAM labeling is applied to measure the absorbed dose of the aqueous solution.
• FAM-labeled oligonucleotides are suited for real-time cell trajectory detection.

Advantages

• FAM-labeled oligonucleotides have high sensitivity, good selectivity, and low detection limits in the process.
• FAM-labeled oligonucleotides have excellent quantum yield and good water solubility.

CD BioGlyco is a biological company specializing in innovative technologies, with perfect cooperation with customers in many countries. At our company, we provide other labeling services for TET, HEX, Cy3&Cy5, TAMRA, and Dabcyl. If you would like to know more, please feel free to contact us.