The Basic Roles of HEX Labeling

HEX, a popular orange dye, is commonly used as a probe for routine PCR as well as for quantitative and multiplex RT-PCR, for measuring changes in fluorescence, or for other applications in which HEX-odn interacts with biopolymers. In particular, HEX labeling is used for so-called multicolor combinatorial probe coding. HEX defines one of 13-15 possible DNA targets in a single PCR, the desired number of probes are labeled with one or 2-4 different fluorophores. Oligodeoxyribonucleotides (ODNs) containing Fluorophores are widely used in molecular biology and medical diagnostics, they are used as probes in real-time PCR (RT-PCR) DNA analysis.

Fig.1 Schematic representation of HEX molecular structure and image in STM. (Kawahara, et al., 2005)

HEX Labeling Service at CD BioGlyco

At CD BioGlyco, we provide high-quality HEX oligonucleotide labeling services. Any post-ammonolysis mixture of HEX-labeled oligodeoxyribonucleotides contained more than 25% hexachloroaryl acridine derivatives. The acridine by-product is not easily detectable or removable, and it may significantly distort the results of quantitative fluorescence analyses using HEX probes.

• We use a new, simple, and reliable post-synthesis deblocking method for HEX-labeled oligonucleotides. Designed to enable high yields of these fluorescent probes and to guarantee the absence of acridine by-products. Under PCR-like analog conditions, we significantly converted the hexachloroaryl acridine derivatives into non-fluorescent derivatives. Each step of the process was strictly controlled. Our company has always been well received by customers with quality service and reliable products.
• The HEX-labeled oligonucleotides were observed using the Scanning Tunneling Microscopy (STM) constant current mode, and then the samples were investigated using scanning tunneling spectroscopy (STS) to evaluate the performance, to ensure the best performance product is provided to our customers. Post-synthetic HPLC purification of HEX-labeled oligonucleotides is essential, where sensitivity or fluorescent signal differentiation (e.g., in quantitative and multiplexed assays) are critical.

Fig.2 Flowchart of HEX labeling inspection service. (CD BioGlyco)

Applications of HEX Labeling

• HEX-labeled oligonucleotides play an important role in the research of medical diagnosis and molecular biology.
• HEX-labeled oligonucleotides can be used in the development of bio-conjugated samples.
• HEX-labeled oligonucleotides can be used as part of a bimolecular probe.
• HEX-labeled oligonucleotides can be used for DNA sequencing and spectroscopy.

CD BioGlyco has been pursuing innovation and progress in the field of Carbohydrate Synthesis, and we constantly developing new processes to increase its applications. Furthermore, we provide efficient and accurate Fluorescein Labeling, Carboxyfluorescein (FAM) Labeling, Tetrachlorofluorescein (TET) Labeling, Cy3&Cy5 Labeling, Dabcyl Labeling, TAMRA Labeling, and BHQ Labeling services. If you are interested in our service, please feel free to contact us further to introduce the specific service in detail.