OPME-based NDP-sugar Synthesis Service

• OPME strategy at CD BioGlyco

The OPME synthesis strategy streamlines multistep enzymatic reactions, capitalizing on the inherent precision of enzyme-catalyzed processes. This approach reorganizes complex de novo biosynthesis into a cascading sequence for efficient target sugar nucleotide production, prioritizing enhanced reaction cleanliness. By identifying high-activity sugar nucleotide synthetases, we aim to enable practical and efficient complex de novo bioconversions, paving the way for synthetic applications.

• NDP-sugar synthesis service at CD BioGlyco

The OPME strategy used at CD BioGlyco seamlessly integrates enzymes responsible for sugar nucleotide donor biosynthesis with glycosyltransferases in a single reaction vessel. This streamlined approach facilitates the efficient synthesis of target glycans from basic monosaccharides and acceptors. Our OPME strategy optimizes NDP-sugar synthesis, enabling the simultaneous and efficient execution of multiple enzymatic steps. This methodology minimizes the requirement for intermediate purifications, enhancing overall yield and ensuring a clean and effective production process. CD BioGlyco also provides traditional Nucleoside & Nucleotide Synthesis Services including Custom Oligonucleotide Synthesis and Polynucleotide Synthesis. Our Modification Services offer a powerful tool for our clients to further meet your requirements. Our series of Nucleotide-based Production Services also be your go-to choice for producing Custom Sugar-nucleotides.

Fig.1 NDP derivatives. (CD BioGlyco)

Here are some of the NDP-sugar products (Fig.2) that we have developed and we are looking forward to working for you to explore more potential NDP-sugars. Our standard synthesis process can be divided into five steps.

• Substrate activation
  -Activation of the sugar substrate and nucleotide triphosphate (NTP) to form an activated intermediate.
• Glycosyl transfer
  -Transfer of the activated sugar moiety from the activated intermediate to the growing NDP-sugar chain.
• Enzyme cascade
  -Integration of multiple enzymes in a sequential cascade to catalyze various reactions, such as glycosyl transferases, nucleotide sugar biosynthetic enzymes, and other relevant enzymes.
• Cofactor regeneration
  -Recycling of cofactors (e.g., NADH, NADPH) to sustain the enzymatic reactions.
• Product generation
  -Generation of the final NDP-sugar product.

Fig.2 OPME synthesis of NDP-sugars. (CD BioGlyco)

Publication Data

Applications

• NDP-sugars serve as donors in glycosylation reactions, where sugar moieties are transferred to acceptor molecules by glycosyltransferase enzymes.
• NDP-sugars are key intermediates in the biosynthesis of polysaccharides such as cellulose, starch, and glycogen.
• NDP-sugar-dependent enzymes are potential targets for drug development.

Advantages

The OPME approach allows for the efficient and simultaneous execution of multiple enzymatic steps, reducing the need for intermediate purifications and enhancing the overall yield of the desired NDP-sugar. This strategy is advantageous for the synthesis of complex sugars with applications in various biological processes.

CD BioGlyco is a leading biotechnology firm specializing in glycan-related solutions. Our lies in Custom Sugar-nucleotides Synthesis, offering tailored services to meet unique research and development needs. With cutting-edge technology and a commitment to precision, CD BioGlyco is your trusted partner for advancing glycan research. Please feel free to contact us for more details.