Custom Oligonucleotide Synthesis Service
Overview
Oligonucleotides are usually composed of up to 20 short-chain nucleotides (deoxyribonucleotides or ribonucleotides). They have a variety of primer lengths. It can be used in pre-designed, customized, or randomized forms for applications in microarrays, gel migration analysis, artificial gene synthesis forensic medicine, etc. There are many types of current research, such as tiny ribonucleic acids, small interfering ribonucleic acids, nucleic acid aptamers, etc. They can achieve very high selectivity by pairing with DNA, mRNA, or pre-mRNA. Based on this, oligonucleotide has a variety of uses. It can be used in diagnostic tests, antisense agents in target validation, competitive inhibitors of transcription factors, and so on. It can also be used as a stimulator of the immune system or as a biotherapeutic agent for the treatment of diseases. In addition, it can precisely inhibit certain genes, silencing genes that code for abnormalities, thus preventing the expression of many faulty proteins.
Key Technologies
At CD BioGlyco, our key technologies for custom oligonucleotide synthesis center on advanced chemical synthesis using optimized stepwise nucleotide addition protocols and enzymatic synthesis for efficient fragment assembly, complemented by four specialized purification methods (OPC columns, HPLC, desalting, PAGE) to ensure high-purity natural or modified oligonucleotides. We achieve precision through real-time reaction monitoring and enzymatic fragment joining.
Your Sequence, Our Science: Custom Oligos Perfected.
CD BioGlyco has excellent synthetic capabilities to meet a variety of custom synthesis needs. Synthetic oligonucleotides avoid the disadvantages of the natural. Due to some shortcomings of the traditional solid-phase methods, such as limited length and purity, scientists began to look for new synthesis methods. At present, we mainly use two methods of chemical synthesis and biological enzyme synthesis to produce.
Fig.1 Oligonucleotide R&D services. (CD BioGlyco)
- Oligonucleotide Synthesis Services
Oligonucleotides are usually prepared by: stepwise addition of nucleotide residues to produce a particular sequence. We synthesize various products using both chemical and biological methods. With advanced synthesis technology and laboratory equipment, we are capable of providing products of various specifications. We produce not only unmodified, standard products but also complex and various modified products based on our Oligonucleotide Modification Service.
Our primary method for oligonucleotide synthesis is solid-phase chemistry using phosphoramidites. This highly successful approach allows for the sequential addition of nucleotides to a growing chain anchored to a solid support, such as a CPG bead. The solid-phase method is highly efficient, scalable, and forms the basis for our high-throughput manufacturing.
For certain specialized applications requiring specific modifications or synthesis scales, we also offer liquid-phase synthesis, a method that can be advantageous for producing specific types of complex oligonucleotides.
We are at the forefront of exploring new technologies. While chemical synthesis is our current standard, we are actively developing and optimizing enzymatic synthesis methods, which are gaining traction as a greener and potentially more efficient alternative for certain applications.
We provide a tiered range of purification options to ensure the final product meets the specific purity requirements of your application.
This is the standard purification method, sufficient for most applications like standard PCR. It removes small molecule contaminants such as organic salts generated during the synthesis and deprotection processes.
This is a step up from desalination, providing a higher level of purity. It is ideal for applications that require a cleaner product than what desalting provides but do not demand the precision of HPLC or PAGE.
HPLC is the method of choice for purifying oligonucleotides that are modified with non-standard bases, fluorescent dyes, or linkers. Depending on the modification, we offer both reverse-phase and ion-exchange HPLC to separate the full-length product from truncated species based on differences in solvent affinity or charge.
PAGE is the most efficient method for isolating full-length, unmodified oligonucleotides from shorter failure sequences. This method is particularly useful for longer oligonucleotides intended for applications like cloning, where a high level of purity is critical.
Workflow
Publication Data
DOI.: 10.1038/s42004-024-01216-0
Journal: Communications Chemistry
Published: 2024
Results: This perspective reviews controlled enzymatic synthesis (CES) as an emerging method for producing oligonucleotides, contrasting it with traditional chemical synthesis. The authors highlight how CES uses template-independent polymerases (like terminal deoxynucleotidyl transferase) to iteratively add nucleotides to a primer immobilized on a solid support. Crucially, each nucleotide is temporarily blocked (e.g., at the 3'-OH position) to ensure single-base additions per cycle, followed by deblocking. The review details advancements in designing reversible blocking groups and engineered polymerases for efficient DNA synthesis. It also examines significant challenges for extending CES to RNA and xenonucleic acids due to polymerase compatibility and suitable blocking strategies. The authors conclude by suggesting that combining CES with ligase-based assembly could overcome current length limitations and enable the production of complex, modified oligonucleotides.
Applications of Custom Oligonucleotide Synthesis
- Plant chromosomes can be studied using the developed oligonucleotide probes. The study of plant chromosomes can ultimately provide a cytological basis for elucidating plant classification, origin, evolution, phylogeny, genetics, and so on.
- The occurrence of cancer is closely related to the abnormal regulation of signaling pathways, etc. Antisense oligonucleotide molecules are used to study the effects of cancer growth, metastasis, etc., in vitro and in vivo.
- Oligonucleotide primers can be used for gene cloning, sequencing, etc. Through its inhibition or silencing of gene expression enables screening for genetic diseases or identification of specific pathogens.
Advantages of Us
- We have excellent chemical and enzymatic synthesis capabilities. We monitor chemical reactions in real time to ensure optimal product quality.
- Enzymatic methods offer several advantages. Enzymatic reactions allow small fragments to be joined to become large fragments, thus speeding up the synthesis process.
- Oligonucleotides have a wide variety of biological applications, etc. Both natural and modified oligonucleotide products can be synthesized.
Frequently Asked Questions
Associated Services
Our oligonucleotide synthesis is complemented by related associated services for specific modifications:
- Affinity-plus Base Modification
- Inosine Modification
- 2'-O-methoxy-ethyl Bases (2'-MOE) Modification
- 2'-O-Methyl RNA Bases Modification
- Fluoro Bases Modification
CD BioGlyco aims to facilitate drug research, molecular probe assay development, and so on. We also provide production services such as Polynucleotide and Nucleoside-based Production. To learn more about our products and how to synthesize them, please feel free to contact us.
Reference
- Pichon, M.; Hollenstein, M. Controlled enzymatic synthesis of oligonucleotides. Communications Chemistry. 2024, 7(1): 138. (Open Access)
