Influenza A virus contains two main viral proteins on its surface: neuraminidase (NA) and haemagglutinin (HA). It also contains the ion channel protein M2 on its surface. The main function of HA is to bind to the sialic acid receptor on the surface of the host cell membrane and help the viral envelope to fuse with the host cell membrane. The main function of NA is to mediate the release of nascent viral particles assembled in the host cell by cleavage of the sialic acid moiety in the glycoproteins. Both HA and NA play key roles in virus-receptor binding. Vaccination is a terrific preventive measure against influenza. Currently, influenza vaccines are mainly inactivated, live attenuated, and recombinant vaccines. HA and NA recombinant glycoprotein vaccines are a hot research topic and have good immunogenicity. It is found that the chimeric HA (cHA) vaccine elicits broadly protective CD8+ and CD4+ T cell responses.
Fig.1 The structure of influenza A virus. (Wikipedia)
CD BioGlyco provides high-quality sugar-related Vaccine Development Services to clients. HA, NA, and nucleoprotein (NP) are the three major antigens of influenza viruses and play a vital role in the virus life cycle. Their recombinant proteins are widely used in several fields. We establish advanced experimental platforms for the design, production, and evaluation of HA and NA Recombinant Glycoprotein Vaccines, etc.
Vaccines made from genetically engineered protein antigens can be used to replace subunit vaccines produced by conventional methods. More importantly, it can be used in vaccine research for viruses that cannot be easily cultured. These vaccines are safe. Its immunogenicity can be further enhanced by the use of Adjuvants. We express HA and NA proteins in a variety of systems, such as Yeast Systems, Mammals, insect cells, etc. After protein expression, we purify and characterize them. We optimize the purification methods for HA and NA. The optimized purification method is convenient and fast, which is suitable for vaccine production. We have a comprehensive vaccine evaluation system. The safety and immunology of the vaccine can be tested after purification.
We also construct different forms of HA and NA recombinant proteins using HA or NA as target antigens. Then we compare and screen the best forms of protein vaccines based on HA and NA.
Fig.2 The process of vaccine development. (CD BioGlyco)
Technology: Gene Editing Technology
Journal: Proceedings of the National Academy of Sciences of the United States of America
IF: 11.205
Published: 2020
Results: In this study, chimeric HA vaccines against several influenza A viruses were prepared. Their immune prototypes were tested by mouse experiments. The results showed that the chimeric H5/1 vaccine induced stronger CD8+ cell responses compared with the exchange H5/1 vaccine. The immunogenicity of chimeric H5/1 vaccines with different glycosylation status was also investigated in this study. The results showed that monoglycosylated cHA (cHA mg) induced better antibody responses compared with fully glycosylated cHA vaccine.
Fig.3 Construction of chimeric vaccines and vaccination-induced antibody testing. (Liao, et al., 2020)
CD BioGlyco, a leading global biologic company, has a high level of competence in the development of sugar-related vaccines. Our diverse R&D and technical capabilities will help you accelerate progress in the development of sugar-related vaccines. Feel free to contact us for more detailed information on vaccine development. We will be the first to address all your questions about glycoprotein vaccine development.
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