Custom Oligosaccharides Synthesis
Overview
CD BioGlyco has unique technology platforms and powerful analysis tools to provide customers with high-quality custom services to synthesize oligosaccharides. We have confidence to be your essential research assistant in the field of glycobiology.
Key Technologies
Solid-phase synthesis; Stereospecific glycosylation
Synthesis Simplified, Complexity Delivered
In general, carbohydrates can be divided into two categories: monosaccharides and complex carbohydrates. When two or more monosaccharide units are connected through glycosidic bonds, complex carbohydrates are formed. Oligosaccharides are usually polymers of 3 to 10 monosaccharides, which usually exist in the form of glycans: oligosaccharide chains linked to lipids or amino acid side chains in proteins. Oligosaccharides have many functions, including cell recognition and cell binding. These biological processes involving carbohydrates are usually not related to monosaccharides, but the structure of oligosaccharides in glycoconjugates. Therefore, oligosaccharides are becoming more important in the study of biological activity.
However, in experiments, it is almost impossible to completely separate homogeneous glycoform, and the conversion of glycosylation to clinical applications in humans involves systematic studies using homogeneous samples of oligosaccharides and glycoconjugates. In the past two decades, the understanding of glycobiology has made great strides. These advances are partly attributed to the latest developments in oligosaccharides synthesis, mainly the synthesis of human-type sequences to assist biomedical research. Oligosaccharides can be synthesized by chemical, enzymatic or other biological methods. Complex oligosaccharides synthesis requires a combination of multiple disciplines, including practical synthesis methods and effective tools in cell and molecular biology, biochemistry and glycoproteomics.
Fig 1. Example: human milk oligosaccharides basic structures (Plaza-Díaz, J.; et al. 2018)
Discover Our Services
The structural complexity and wide-range variations of glycans and their conjugates represent the main challenges in the synthesis of such biomolecules. To help customers better research oligosaccharides, CD BioGlyco provides two main methods, chemical synthesis and enzymatic synthesis, to obtain homogeneous oligosaccharides and glycoconjugates.
- Chemical synthesis has always been an exploratory research method as this mature discipline can provide access to almost any structure.
- Enzymatic synthesis can be carried out stereo- and regiospecifically without protecting group manipulations. Compared with chemical glycosylation, the reaction conditions of enzyme-catalyzed glycosylation are extremely mild. Usually enzymatic or chemoenzymatic methods are used for process optimization.
| Custom Cyclodextrin Development | High-performance Liquid Chromatography-Charged Aerosol Detection (HPLC-CAD)-based Oligosaccharides Analysis Service |
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| We provide tailored cyclodextrin development for both Pharmaceutical applications and Non-pharmaceutical uses, employing precise chemical modifications, supported by comprehensive Analysis and characterization. | Utilize CAD coupled with HPLC for universal, label-free quantification and purity assessment of diverse oligosaccharides, independent of chromophore presence. |
| Size Exclusion Chromatography (SEC)-based Oligosaccharides Analysis Service | Polysaccharide Fluorescent Labeling Service |
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| Determine molecular weight distribution, purity, and aggregation state of oligosaccharides and glycoconjugates using high-resolution SEC separation. | Enhance detection sensitivity for complex polysaccharides via covalent attachment of fluorescent tags, enabling advanced analysis by HPLC-fluorescence detection (FLD) or capillary electrophoresis-laser-induced fluorescence (CE-LIF), particularly for trace-level samples. |
With a professional synthesis platform, CD BioGlyco provides our clients with scientific assistance custom cyclodextrin development. Particularly, we provide several strategies to prepare cyclodextrins-guest complexes including kneading, grinding, microwave irradiation, supercritical carbon dioxide, co-precipitation, and spray-dried.
Publication Data
DOI.: 10.1038/s41467-020-17778-1
Journal: Nature communications
IF: 14.7
Published: 2020
Results: The authors developed and validated a novel non-enzymatic chemical method called FITDOG to universally cleave diverse polysaccharides into representative oligosaccharides suitable for structural analysis. Recognizing the lack of a universal enzymatic equivalent to trypsin for polysaccharides due to their immense structural complexity and heterogeneity, FITDOG uses iron-catalyzed oxidation (Fe3+/H2O2) under controlled conditions to depolymerize polysaccharides without bias toward specific linkages or structures. The resulting oligosaccharides, typically with degrees of polymerization (DP) of 3-14, retain key structural features of the parent polymer and can be effectively analyzed using liquid chromatography-mass spectrometry (LC-MS) for applications like polysaccharide identification ("oligosaccharide fingerprinting") in complex mixtures (e.g., food, feces) and de novo structural characterization, as demonstrated for various standards and in a study tracking dietary polysaccharide fate in an infant gut microbiome. This method overcomes a major bottleneck in glycobiology by enabling high-sensitivity, high-throughput structural analysis of polysaccharides.
Workflow
Applications
- Designing and synthesizing novel oligosaccharide mimics or derivatives that can act as inhibitors of pathogen adhesion (e.g., bacterial, viral), modulators of immune responses, or anti-cancer agents.
- Using customized glycans to identify and validate new drug targets by probing their interactions with receptors or enzymes involved in disease pathways.
- Designing and synthesizing glycan-functionalized biomaterials to modulate cell adhesion, differentiation, and tissue regeneration, mimicking the extracellular matrix.
Advantages of Us
- Detailed analysis of the synthesized molecules to ensure the high quality of the final product
- Highly integrated approaches can combine chemical and enzymatic methods to quickly assemble oligosaccharides
- Update methods according to international development
- Customized route design
Frequently Asked Questions
Associated Services
CD BioGlyco fully combines chemical synthesis and enzymatic synthesis to provide customers with high-quality synthetic oligosaccharides. The comprehensive post-synthesis analysis experiment provides a sufficient guarantee for customers' products.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.
References
- Plaza-Díaz, J.; et al. Human milk oligosaccharides and immune system development. Nutrients. 2018, 10(8): 1038. (Open Access)
- Amicucci, M.J.; et al. A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis. Nature Communications. 2020, 11(1): 3963. (Open Access)
