DNA and RNA Purification Service
The uses of synthetic DNA and RNA are expanding across various fields, including clinical diagnostics and the development of novel biopharmaceutical therapeutics. DNA and RNA synthesis is a highly efficient process that generates minimal impurities at each step during the synthesis cycle. For example, it is crucial to purify RNAs obtained from enzymatic synthesis or modification reactions before utilization. After in vitro transcription, the RNA needs to be purified to remove unincorporated nucleotides, short aborted transcripts, enzymes, and buffer components. After performing protocols like RNA labeling, DNase I treatment, proteinase K treatment, and mRNA capping, it is crucial to eliminate small molecule and enzyme reaction components. This thorough removal to produce highly purified RNA is necessary for further applications or experiments, such as RNA vaccines.
Fig.1 Types of mRNA vaccines. (Esteban, et al., 2021)
The key technologies of our DNA and RNA purification service include PAGE-based purification, which separates polynucleotides by size with high resolution, and HPLC-based purification employing three specialized methods: reversed-phase HPLC (separates by hydrophobicity), ion-exchange HPLC (separates by charge), and ion-paired reversed-phase HPLC (enhances resolution for charged molecules), ensuring high-purity outcomes suitable for sensitive downstream applications.
This purified DNA and RNA can be used for diverse downstream applications. As a result, CD BioGlyco offers better methods to purify and analyze these vital biological aptamers to guarantee delivering DNA and RNA of high quality. We have two techniques available to purify and isolate DNA and RNA from synthetic products. These techniques are employed to eliminate reaction by-products and undesired oligonucleotides produced during the synthetic reactions. We primarily utilize PAGE and HPLC for the acquisition of desired compounds derived from synthetic products. We efficiently isolate the desired DNA and RNA and remove any unwanted by-products and impurities that may arise during the synthetic reactions through our techniques.
Fig.2 The main factors determining the separation properties. (CD BioGlyco)
Our streamlined purification workflow is designed for maximum efficiency, transparency, and quality assurance. Each project undergoes a meticulous, multi-stage process to ensure the highest possible nucleic acid yield and integrity.
The process begins with the careful receipt and preparation of your samples. We employ a combination of physical, chemical, and enzymatic methods to disrupt cell membranes and release nucleic acids into a solution, creating a crude lysate.
The crude lysate is then processed to remove cellular debris, lipids, and proteins that could interfere with subsequent purification steps. This is a critical stage that ensures the integrity of the downstream purification process.
Based on your project requirements, we apply either our PAGE or HPLC purification protocols. For PAGE, we first visualize the DNA or RNA band of interest, carefully excise it from the gel, and then recover the nucleic acid from the gel matrix. For HPLC, the sample is injected into a column where it binds to a resin and is then eluted in a stepwise manner to separate the target molecules.
After purification, the target nucleic acids are washed to remove residual salts, buffers, and other contaminants. The final, highly purified DNA or RNA is then eluted in a low-ionic-strength solution, such as nuclease-free water, to ensure its readiness for your specific downstream application.
Every purified sample undergoes rigorous quality control. We provide comprehensive reports detailing the concentration, purity, and integrity of your nucleic acids, giving you a complete overview of the quality of your sample.
DOI.: 10.3390/ijms17122134
Journal: International Journal of Molecular Sciences
IF: 4.9
Published: 2016
Results: This comprehensive review details advanced methods for purifying and determining the structures of therapeutic oligonucleotides and aptamers. The authors systematically analyze purification techniques—including PAGE, ion-exchange chromatography, and ion-paired reversed-phase HPLC—highlighting their efficacy in isolating homogeneous samples for structural studies. For structure determination, the paper evaluates X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, emphasizing solutions to challenges like crystallization bottlenecks and resonance overlap in NMR. Key innovations include selenium-derivatized nucleic acids for crystallographic phasing and novel NMR experiments for dynamic analysis. The integration of these methodologies enables precise structural insights critical for optimizing aptamer-based therapeutics and diagnostics.
Our DNA and RNA purification delivers high-integrity nucleic acids essential for genomics and transcriptomics studies. To unlock deeper insights into glycan-mediated biological recognition, critical for understanding infection, immunity, and cellular communication, we provide advanced Glycan Microarray Assays. These platforms rapidly profile interactions between carbohydrates and diverse biomolecules (e.g., antibodies, lectins, viruses), enabling high-throughput functional glycomics:
As a leading company in the field of glycobiology, CD BioGlyco excels in DNA and RNA purification. We possess several advantages that set us apart from others. We employ state-of-the-art purification techniques to ensure efficient and pure extraction of the target molecules. Besides, our research team possesses extensive knowledge and experience in the field. If you would like to know more about our services, we encourage you to contact us for further information.
References
