PAGE-based DNA and RNA Purification Service
DNA and RNA are a class of macromolecules with important functions for living organisms, and there is often a requirement for high purity for their use. Therefore, it is a very urgent matter to comprehensively study the way of separating and purifying biomolecules. Among the techniques for the separation and purification of biological macromolecules, gel electrophoresis is a basic technique in the laboratory of biological disciplines. This technique allows efficient separation of macromolecules such as DNA, RNA, and proteins. For proteins in particular, polyacrylamide gel electrophoresis (PAGE) is often the technique of choice. The separation principle of PAGE is based on the electrophoretic mobility of biomolecules (the ability of an analyte to move toward an electrode of opposite charge). In the use of PAGE, the charge, size (molecular weight), and shape of the molecule determine the specific operation.
The key technology of this service is PAGE, which utilizes an electric field to separate DNA and RNA fragments based on their electrophoretic mobility, influenced by size, charge, and shape, under denaturing conditions for high-resolution purification and subsequent extraction of target molecules from the gel.
For the use of PAGE purification technology, CD BioGlyco has many years of operational experience. The PAGE purification method we offer mainly utilizes denaturing polyacrylamide gel electrophoresis for the separation of primed DNA / RNA, followed by the recovery of the target DNA / RNA from the gel. Currently, the PAGE purification method we offer provides greater than 90% purity of purified DNA and is particularly effective for the purification of long-stranded DNA (greater than 50 mer).
The PAGE purification technique is suitable for the isolation and purification of large polynucleotide DNA or RNA fragments and allows the isolation of relatively small proteins.
Fig.1 Flow chart of PAGE purification technology. (CD BioGlyco)
DOI.: 10.1021/acs.oprd.4c00382
Journal: Organic Process Research & Development
IF: 3.5
Published: 2024
Results: This review details PAGE as a traditional method for purifying synthetic oligonucleotides based on length/size separation. PAGE effectively isolates full-length products from failure sequences (e.g., n–1 impurities) by exploiting differential migration through a gel matrix under an electric field. While advantageous for analytical and low-preparative scales (≤1 mmol), PAGE exhibits critical limitations: low recovery rates (<50%) due to post-electrophoresis extraction and desalting steps, inability to resolve chemically modified nucleosides (e.g., 2′-OMe, 2′-F), and poor scalability. The method is also labor-intensive and time-consuming, requiring extensive manual handling. Despite its historical utility for research-grade oligonucleotides, PAGE is largely superseded by chromatographic techniques (e.g., ion-exchange, reversed-phase) for therapeutic applications demanding higher purity, yield, and scalability.
Our PAGE-based DNA and RNA purification offers high-resolution size selection for nucleic acid fragments, ideal for applications demanding precise molecular weight isolation—from CRISPR guide RNAs to synthetic biology constructs. To transform these purified sequences into functionally annotated tools for decoding glycan-protein interactions, we offer LiGA Construction Services. This platform genetically engineers multivalent glycan probes for live-cell or in vivo binding studies, enabling:
CD BioGlyco has many years of experimental experience in the purification of DNA and RNA. We have professional researchers and first-class experimental equipment to provide custom isolation and purification solutions for clients all over the world. At the same time, we also provide timely feedback to our clients on the progress of their experiments. If you are interested in our services, please feel free to contact us.
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