Human lipopolysaccharide-binding protein (LBP) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of human LBP as low as 1 μmol/L and its detection range is 7.5-240 μmol/L.
Assay Principle
The kit utilizes purified human LBP antibody to coat microtiter plate wells, creating a solid-phase antibody. The wells are then exposed to LBP samples, resulting in the formation of an antibody-antigen-enzyme-antibody complex when combined with HRP-labeled antibody. After thorough washing, a TMB substrate solution is added, causing the substrate to turn blue due to the catalytic activity of HRP enzyme. The reaction is halted by the addition of a sulfuric acid solution, and the change in color is spectrophotometrically measured at a wavelength of 450 nm. By comparing the Optical Density (O.D.) of the samples to a standard curve, the concentration of LBP in the samples can be determined.
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
7.5-240 μmol/L
Sensitivity
1 μmol/L
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Recovery
>95%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Human lipopolysaccharide-binding protein (LBP) ELISA kit can be used to measure the level of human LBP in serum, plasma, tissue homogenate and other biological fluid samples.
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